Human FASL / CD95LG / CD95 Ligand PicoKine® Quick ELISA Kit

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human FASL / CD95LG / CD95 Ligand PicoKine® Quick ELISA Kit

catalog :

FEK0337

quantity :

96 wells/kit, with removable strips.

price :

499 USD

more info or order :

Boster product webpage

citations: 3

Reference
Alkhouri N, Alisi A, Okwu V, Matloob A, Ferrari F, Crudele A, et al. Circulating Soluble Fas and Fas Ligand Levels Are Elevated in Children with Nonalcoholic Steatohepatitis. Dig Dis Sci. 2015;60:2353-9 pubmed publisher
Grygorczuk S, Osada J, Moniuszko A, ÅšwierzbiÅ„ska R, Kondrusik M, Zajkowska J, et al. Increased expression of Fas receptor and Fas ligand in the culture of the peripheral blood mononuclear cells stimulated with Borrelia burgdorferi sensu lato. Ticks Tick Borne Dis. 2015;6:189-97 pubmed publisher
Ristić T, Djordjevic V, Deljanin Ilic M, Cosic V, Kundalić S. Serum Fas/FasL levels in dependence on clinical presentations of coronary disease and their relationship with risk factors. Vojnosanit Pregl. 2010;67:537-42 pubmed

image

image 1 :

Human FASL PicoKine ELISA Kit standard curve

product information

SKU :

FEK0337

Product Name :

Human FASL / CD95LG / CD95 Ligand PicoKine® Quick ELISA Kit

Size :

96 wells/kit, with removable strips.

Price :

499 USD

Description :

Human FASL / CD95LG / CD95 Ligand PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human FASLG in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 2pg/ml. Introduction: Boster's PicoKine® Quick ELISA kits features the high sensitivity of PicoKine® ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours. Compared to regular Picokine ELISA kits, the following sections in the datasheet are different: 1. kit components; 2. preparation before experiment; 3. assay protocol

Reactivity :

Human

Application :

ELISA

Gene Name :

FASLG

Uniprot ID :

P48023

Immunogen :

Expression system for standard: CHO; Immunogen sequence: P134-L281

Cross-reactivity :

There is no detectable cross-reactivity with other relevant proteins.

Assay Range :

15.6 pg/ml - 1,000 pg/ml

Sensitivity :

<2 pg/ml

Sample Type :

cell culture supernatants, serum and plasma (heparin, EDTA, citrate)

Capture/Detection Antibodies :

monoclonal antibody from mouse, polyclonal antibody from goat

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Gene Full Name :

Tumor necrosis factor ligand superfamily member 6

Synonyms :

Tumor necrosis factor ligand superfamily member 6;Apoptosis antigen ligand;APTL;CD95 ligand;CD95-L;Fas antigen ligand;Fas ligand;FasL;CD178;Tumor necrosis factor ligand superfamily member 6, membrane form;Tumor necrosis factor ligand superfamily member 6, soluble form;Receptor-binding FasL ectodomain;Soluble Fas ligand;sFasL;ADAM10-processed FasL form;APL;FasL intracellular domain;FasL ICD;SPPL2A-processed FasL form;SPA;FASLG;APT1LG1, CD95L, FASL, TNFSF6;

Protein Name :

Tumor necrosis factor ligand superfamily member 6

Molecular Weight :

31485 MW

Protein Function :

Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells (PubMed:26334989, PubMed:9228058). Involved in cytotoxic T-cell-mediated apoptosis, natural killer cell-mediated apoptosis and in T-cell development (PubMed:9228058, PubMed:7528780, PubMed:9427603). Initiates fratricidal/suicidal activation-induced cell death (AICD) in antigen-activated T-cells contributing to the termination of immune responses (By similarity). TNFRSF6/FAS-mediated apoptosis has also a role in the induction of peripheral tolerance (By similarity). Binds to TNFRSF6B/DcR3, a decoy receptor that blocks apoptosis (PubMed:27806260).

Subcellular Localization :

Cell membrane ; Single-pass type II membrane protein . Cytoplasmic vesicle lumen . Lysosome lumen . Is internalized into multivesicular bodies of secretory lysosomes after phosphorylation by FGR and monoubiquitination (PubMed:17164290). Colocalizes with the SPPL2A protease at the cell membrane (PubMed:17557115).

Background :

Fas ligand (FasL or CD95L) is a type-II transmembrane protein that belongs to the tumor necrosis factor (TNF) family. Its binding with its receptor induces apoptosis. The human FASL gene consists of approximately 8 kb and is split into 4 exons. Fas ligand/receptor interactions play an important role in the regulation of the immune system and the progression of cancer. Fas ligand or FasL is a homotrimeric type II transmembrane protein. It signals through trimerization of FasR, which spans the membrane of the "target" cell. This trimerization usually leads to apoptosis, or cell death. Soluble Fas ligand is generated by cleaving membrane-bound FasL at a conserved cleavage site by the external matrix metalloproteinase MMP-7.

more info or order :

Boster product webpage