Anti-human CD13 ANPEP Monoclonal Antibody Unconjugated, Flow Validated

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product summary

company name :

Boster

product type :

antibody

product name :

Anti-human CD13 ANPEP Monoclonal Antibody Unconjugated, Flow Validated

catalog :

FC02591

quantity :

100 ug, 500 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

APN-1464

reactivity :

human

application :

flow cytometry

product information

SKU :

FC02591

Product Name :

Anti-human CD13 ANPEP Monoclonal Antibody Unconjugated, Flow Validated

Size :

100 ug, 500 ug

Clonality :

Monoclonal

Clone Number :

APN-1464

Host :

Mouse

Reactivity :

Human

Application(s) :

Flow Cytometry

Application Details :

Flow Cytometry, 5µl is enough to stain 1 million PBMCs or 100µl whole blood sample.

Description :

Boster Bio Anti-human CD13 ANPEP Monoclonal Antibody Unconjugated, Flow Validated catalog # FC02591. Tested in Flow Cytometry applications. This antibody reacts with Human. Introduction: This antibody is routinely tested by flow cytometric analysis. Flow cytometry and other applications were tested during antibody development by Boster or are reported in the literature.Clone APN/1464 recognizes cell surface CD13 antigen, a 150kDa membrane glycoprotein. The CD13 antigen is highly expressed mostly on myeloid-derived hematopoietic cells including granulocytes, monocytes, mast cells, and GM-progenitor cells. CD13 abundantly expresses on most of the malignant cells of myeloid origin such as AML, CML and also on smaller subset of cancer cells of lymphoid origin. Normal lymphocytes, platelets and erythrocytes do not express CD13. CD13 plays important role in metabolism of biologically active peptides, in phagocytosis, and in bactericidal/tumoricidal immune process. It also serves as a receptor for human coronaviruses (HCV). Application Information: Each lot of this antibody has been quality control tested by flow cytometric analysis using human PBMCs. For flow cytometric staining, the recommended use of this antibody is 0.5µg per 1x106 cells in 100µl of staining volume or 0.1ml whole blood followed by a florescent conjugated anti-mouse secondary antibody. However, it is strongly suggested that the antibody reactivity be empirically titrated for optimal performance in the application of interest.

Concentration :

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Gene Name :

ANPEP

Uniprot ID :

P15144

Form :

Liquid

Contents :

PBS, pH7.2, 0.09% NaN3

Purification :

Protein A purified

Isotype :

IgG1,k

Storage :

Store at 4°C for one year. Do not freeze.

Gene Full Name :

Aminopeptidase N

Synonyms :

Aminopeptidase N; AP-N; hAPN; 3.4.11.2; Alanyl aminopeptidase; Aminopeptidase M; AP-M; Microsomal aminopeptidase; Myeloid plasma membrane glycoprotein CD13; gp150; CD13; ANPEP; APN, CD13, PEPN;

Protein Name :

Aminopeptidase N

Molecular Weight :

109540 MW

Protein Function :

Broad specificity aminopeptidase. Plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. May play a critical role in the pathogenesis of cholesterol gallstone disease. May be involved in the metabolism of regulatory peptides of diverse cell types, responsible for the processing of peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines. Found to cleave antigen peptides bound to major histocompatibility complex class II molecules of presenting cells and to degrade neurotransmitters at synaptic junctions. Is also implicated as a regulator of IL-8 bioavailability in the endometrium, and therefore may contribute to the regulation of angiogenesis. Is used as a marker for acute myeloid leukemia and plays a role in tumor invasion. In case of human coronavirus 229E (HCoV-229E) infection, serves as receptor for HCoV-229E spike glycoprotein. Mediates as well human cytomegalovirus (HCMV) infection.

Subcellular Localization :

Cell membrane; Single-pass type II membrane protein. Cytoplasm, cytosol. A soluble form has also been detected.

Tissue Specificity :

Expressed in epithelial cells of the kidney, intestine, and respiratory tract; granulocytes, monocytes, fibroblasts, endothelial cells, cerebral pericytes at the blood- brain barrier, synaptic membranes of cells in the CNS. Also expressed in endometrial stromal cells, but not in the endometrial glandular cells. Found in the vasculature of tissues that undergo angiogenesis and in malignant gliomas and lymph node metastases from multiple tumor types but not in blood vessels of normal tissues. A soluble form has been found in plasma. It is found to be elevated in plasma and effusions of cancer patients.

Background :

Alanine aminopeptidase, also known as ANPEP or CD13. is an enzyme that is used as a biomarker to detect damage to the kidneys, and that may be used to help diagnose certain kidney disorders. It is mapped to 15q26.1. Aminopeptidase N is located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes. In the small intestine, Aminopeptidase N plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases, and it is also thought to be involved in the metabolism of regulatory peptides by diverse cell types, including small intestinal and renal tubular epithelial cells, macrophages, granulocytes, and synaptic membranes from the CNS. Petrovic et al showed that CD13 was required for endothelial cell invasion in response to bradykinin. Inhibition of CD13 abrogated internalization of bradykinin receptor B2 and reduced endothelial cell motility.

Research Category :

Surface Molecules