Anti-human CD13 ANPEP Monoclonal Antibody PerCP-Cy5.5 Conjugated, Flow Validated | Boster FC02591-PerCP-Cy5.5 product information

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product summary

company name :

Boster

product type :

antibody

product name :

Anti-human CD13 ANPEP Monoclonal Antibody PerCP-Cy5.5 Conjugated, Flow Validated

catalog :

FC02591-PerCP-Cy5.5

quantity :

100 Tests, 200 Tests

clonality :

monoclonal

host :

mouse

conjugate :

PerCP/Cy5.5

clone name :

APN1464

reactivity :

human

application :

flow cytometry

product information

SKU :

FC02591-PerCP-Cy5.5

Product Name :

Anti-human CD13 ANPEP Monoclonal Antibody PerCP-Cy5.5 Conjugated, Flow Validated

Size :

100 Tests, 200 Tests

Clonality :

Monoclonal

Clone Number :

APN1464

Host :

Mouse

Conjugate :

PerCP-Cy5.5

Reactivity :

Human

Application(s) :

Flow Cytometry

Application Details :

User need to optimize the dilution ratio for this antibody

Description :

Boster Bio Anti-human CD13 ANPEP Monoclonal Antibody PerCP-Cy5.5 Conjugated, Flow Validated catalog # FC02591-PerCP-Cy5.5. Tested in Flow Cytometry applications. This antibody reacts with Human. Introduction: Clone APN1464 recognizes cell surface CD13 antigen, a 150kDa membrane glycoprotein. The CD13 antigen is highly expressed mostly on myeloid-derived hematopoietic cells including granulocytes, monocytes, mast cells, and GM-progenitor cells. CD13 abundantly expresses on most of the malignant cells of myeloid origin such as AML, CML and also on smaller subset of cancer cells of lymphoid origin. Normal lymphocytes, platelets and erythrocytes do not express CD13. CD13 plays important role in metabolism of biologically active peptides, in phagocytosis, and in bactericidal/tumoricidal immune process. It also serves as a receptor for human coronaviruses (HCV). This antibody is routinely tested by flow cytometric analysis. Flow cytometry and other applications were tested during antibody development by Boster or are reported in the literature. Application Information: Each lot of this antibody has been pre-titrated and tested by flow cytometric analysis using human PBMCs. 5µl of this product is found sufficient for staining 1 million cells in a 100µl staining volume or 100µl of whole blood. However, it is recommended that antibody reactivity be empirically titrated for optimal performance in the application of interest.

Concentration :

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Gene Name :

ANPEP

Uniprot ID :

P15144

Immunogen :

Recombinant human CD13 protein

Form :

Liquid

Contents :

PBS with 0.09% NaN3 and 0.2% (w/v) BSA

Isotype :

IgG1,k

Storage :

4°C and protect from prolonged exposure to light. Do not freeze.

Gene Full Name :

Aminopeptidase N

Synonyms :

Aminopeptidase N; AP-N; hAPN; 3.4.11.2; Alanyl aminopeptidase; Aminopeptidase M; AP-M; Microsomal aminopeptidase; Myeloid plasma membrane glycoprotein CD13; gp150; CD13; ANPEP; APN, CD13, PEPN;

Protein Name :

Aminopeptidase N

Molecular Weight :

109540 MW

Protein Function :

Broad specificity aminopeptidase. Plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. May play a critical role in the pathogenesis of cholesterol gallstone disease. May be involved in the metabolism of regulatory peptides of diverse cell types, responsible for the processing of peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines. Found to cleave antigen peptides bound to major histocompatibility complex class II molecules of presenting cells and to degrade neurotransmitters at synaptic junctions. Is also implicated as a regulator of IL-8 bioavailability in the endometrium, and therefore may contribute to the regulation of angiogenesis. Is used as a marker for acute myeloid leukemia and plays a role in tumor invasion. In case of human coronavirus 229E (HCoV-229E) infection, serves as receptor for HCoV-229E spike glycoprotein. Mediates as well human cytomegalovirus (HCMV) infection.

Subcellular Localization :

Cell membrane; Single-pass type II membrane protein. Cytoplasm, cytosol. A soluble form has also been detected.

Tissue Specificity :

Expressed in epithelial cells of the kidney, intestine, and respiratory tract; granulocytes, monocytes, fibroblasts, endothelial cells, cerebral pericytes at the blood- brain barrier, synaptic membranes of cells in the CNS. Also expressed in endometrial stromal cells, but not in the endometrial glandular cells. Found in the vasculature of tissues that undergo angiogenesis and in malignant gliomas and lymph node metastases from multiple tumor types but not in blood vessels of normal tissues. A soluble form has been found in plasma. It is found to be elevated in plasma and effusions of cancer patients.

Background :

Alanine aminopeptidase, also known as ANPEP or CD13. is an enzyme that is used as a biomarker to detect damage to the kidneys, and that may be used to help diagnose certain kidney disorders. It is mapped to 15q26.1. Aminopeptidase N is located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes. In the small intestine, Aminopeptidase N plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases, and it is also thought to be involved in the metabolism of regulatory peptides by diverse cell types, including small intestinal and renal tubular epithelial cells, macrophages, granulocytes, and synaptic membranes from the CNS. Petrovic et al showed that CD13 was required for endothelial cell invasion in response to bradykinin. Inhibition of CD13 abrogated internalization of bradykinin receptor B2 and reduced endothelial cell motility.

Research Category :

Cell Type Markers, Hematopoietic Progenitors, Immunology, Lymphoid, Mesenchymal Stem Cells, Myeloid, Stem Cells, Surface Molecules