This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Boster
product type :
antibody
product name :
Anti-human CD8 Cd8a Monoclonal Antibody Unconjugated, Flow Validated
catalog :
FC02236
quantity :
100 ug, 500 ug
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
OKT8

The same clone is also sold as:
reactivity :
human, mouse
application :
flow cytometry
product information
SKU :
FC02236
Product Name :
Anti-human CD8 Cd8a Monoclonal Antibody Unconjugated, Flow Validated
Size :
100 ug, 500 ug
Clonality :
Monoclonal
Clone Number :
OKT8
Host :
Mouse
Reactivity :
Human
Application(s) :
Flow Cytometry
Application Details :
User need to optimize the dilution ratio for this antibody
Description :
Boster Bio Anti-human CD8 Cd8a Monoclonal Antibody Unconjugated, Flow Validated catalog # FC02236. Tested in Flow Cytometry applications. This antibody reacts with Human. Introduction: The clone OKT‐8 is a 32‐34kDa type I transmembrane glycoprotein and a member of the immunoglobulin superfamily.  The OKT‐8 binds with CD8α forms either a homodimer (2 alpha chains) or a heterodimer with CD8β  and is expressed on the surface of cytotoxic T cells, NK cells, cortical thymocytes, and also on some dendritic cells. CD8 functions as a co‐receptor for T cell receptor and specifically binds to class I MHC for antigen recognition and T cell activation.  This antibody is routinely tested by flow cytometric analysis. Flow cytometry and other applications were tested during antibody development or are reported in the literature. Application Information: Each lot of this antibody has been quality control tested by flow cytometric analysis of human PBMCs.  For flow cytometric staining, the recommended use of this antibody is ≤ 0.5µg per 1x106 cells in 100µl of staining volume followed by a secondary florescent conjugated anti‐mouse antibody.  It is strongly suggested that the antibody reactivity be empirically titrated for optimal performance in the application of interest.
Concentration :
0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
Gene Name :
Cd8a
Uniprot ID :
P01731
Form :
Liquid
Contents :
PBS, pH7.2, 0.09% NaN3
Purification :
Protein A purified
Isotype :
IgG2a,k
Storage :
Store at 4°C for one year. Do not freeze.
Gene Full Name :
T-cell surface glycoprotein CD8 alpha chain
Synonyms :
T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein Lyt-2; CD8a; Cd8a; Lyt-2, Lyt2;
Protein Name :
T-cell surface glycoprotein CD8 alpha chain
Molecular Weight :
27456 MW
Protein Function :
Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.
Subcellular Localization :
Membrane; Single-pass type I membrane protein.
Background :
The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. It is mapped to 2p11.2. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene also encodes the CD8 alpha chain isoforms.
Research Category :
Adaptive Immunity, Immunology, T Cells
company information
Boster
3942 B Valley Ave
Pleasanton, CA 94566
boster@bosterbio.com
https://www.bosterbio.com
925.485.4527
headquarters: USA
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