Anti-human CD2 Monoclonal Antibody Unconjugated, Flow Validated

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-human CD2 Monoclonal Antibody Unconjugated, Flow Validated

catalog :

FC00570

quantity :

100Âµg, 500Âµg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

OKT11

reactivity :

human

application :

flow cytometry

product information

sku :

FC00570

status :

Enabled

name :

Anti-human CD2 Monoclonal Antibody Unconjugated, Flow Validated

category name :

Primary Antibodies, Monoclonal Antibodies, Flow Cytometry Antibodies

gene name :

CD2

price various sizes :

100ug / $79 500ug / $320

clonality :

Monoclonal

clone number :

OKT11

concentration :

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

conjugate :

contents :

PBS, pH7.2, 0.09% NaN3

description :

Monoclonal clone# G2 antibody for CD2 detection. Host: Mouse.Size: 100Âµg, 500Âµg. Tested applications: Flow Cytometry. Reactive species: Human. CD2 information: Molecular Weight: 39448 MW; Subcellular Localization: Membrane; Single-pass type I membrane protein.

short description :

Mouse Monoclonal Anti-human CD2 Unconjugated, designed for Flow Cytometry and validated by Flow Cytometry using Human cells.

description1 :

Introduction . CD2 is a 50kD type I transmembrane glycoprotein and a member of the immunoglobulin superfamily. It is a specific marker for T lymphocytes, natural killer cells, thymic B cell subsets, and some antigen presenting cells. CD2 interacts with CD58 (LFA-3) and CD48 to mediate cell adhesion and induce co-stimulatory signals in T lymphocytes. . This antibody is routinely tested by flow cytometric analysis. Flow cytometry and other applications were tested during antibody development by CapricoBio or are reported in the literature. Application Information . Each lot of this antibody has been quality control tested by flow cytometric analysis of human PBMCs. For flow cytometric staining, the recommended use of this antibody is 0.5ug per 1x106 cells in 100ul of staining volume. It is strongly suggested that the antibody reactivity be empirically titrated for optimal performance in the application of interest.

size :

100Âµg, 500Âµg

uniprot id :

P06729

host :

Mouse

form :

Liquid

purification :

Protein A purified

storage :

4 C . Do not freeze.

isotype :

IgG1, k

sensitivity :

5ul per sample

application details :

User need to optimize the dilution ratio for this antibody

applications :

Flow Cytometry

reactivity :

Human

image labels :

Lymphocytes gated PBMCs stained with purified antiâ€human CD2 (clone OKT 11) (red histogram) followed by Goat antiâ€ mouse IgG (H+L) Alexa Fluor 488. PBMCs stained with only Goat antiâ€mouse IgG (H+L) Alexa Fluor 488 (Blue histogram).

background :

CD2(cluster of differentiation 2) is a cell adhesion molecule found on the surface of T cells and natural killer(NK) cells. It has also been called T-cell surface antigen T11/Leu-5, LFA-2, LFA-3 receptor, erythrocyte receptor and rosette receptor. Monoclonal antibodies directed against CD2 inhibit the formation of rosettes with sheep erythrocytes, indicating that CD2 is the erythrocyte receptor or is closely associated with it. It is one of the earliest T-cell markers, being present on more than 95% of thymocytes; it is also found on some natural killer cells but not on B lymphocytes. Due to its structural characteristics, CD2 is a member of the immunoglobulin superfamily; it possesses two immunoglobulin-like domains in its extracellular portionThe localization of CD2 to 1p13 was established by in situ hybridization. By Southern blotting of DNA from a panel of somatic cell hybrids, Clayton et al.(1988) assigned the CD2 gene to human chromosome 1 and murine chromosome 3.CD2 interacts with other adhesion molecules, such as lymphocyte function-associated antigen-3(LFA-3/CD58) in humans, or CD48 in rodents, which are expressed on the surfaces of other cells. With the use of transgenic mice, such an LCR was identified within the 3-prime flanking region of the human CD2 gene.

competitor equivalent skus :

sc 28807 sc 19639 sc 53038 sc 53036 sc 13531 sc 20046 sc 65247 sc 19640 sc 81732 sc 19638 sc 7318 sc 28808 sc 365017 sc 6987 sc 136974 sc 18907 sc 53823

research category :

Adaptive Immunity, Hematopoietic Progenitors, Immunology, Lymphoid, Myeloid, Stem Cells, T Cells, T Lymphocytic Lineage

synonyms :

T-cell surface antigen CD2;Erythrocyte receptor;LFA-2;LFA-3 receptor;Rosette receptor;T-cell surface antigen T11/Leu-5;CD2;CD2;SRBC;

gene full name :

T-cell surface antigen CD2

molecular weight :

39448 MW

protein function :

CD2 interacts with lymphocyte function-associated antigen (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T- cells, the cytoplasmic domain is implicated in the signaling function.

subcellular localization :

Membrane; Single-pass type I membrane protein.

protein name :

T-cell surface antigen CD2

last modified :

2/6/19 22:57