Anti-human CD45 Monoclonal Antibody FITC Conjugated, Flow Validated

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product summary

company name :

Boster

product type :

antibody

product name :

Anti-human CD45 Monoclonal Antibody FITC Conjugated, Flow Validated

catalog :

FC00555-FITC

quantity :

25 Tests, 100 Tests, 200 Tests

clonality :

monoclonal

host :

mouse

conjugate :

FITC

clone name :

F10-89-4

reactivity :

human

application :

flow cytometry

product information

sku :

FC00555-FITC

status :

Enabled

name :

Anti-human CD45 Monoclonal Antibody FITC Conjugated, Flow Validated

category name :

Primary Antibodies, Monoclonal Antibodies, Flow Cytometry Antibodies

gene name :

PTPRC

price various sizes :

25 Tests / $55 100 Tests / $116 200 Tests / $198

clonality :

Monoclonal

clone number :

F10-89-4

concentration :

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

conjugate :

FITC

contents :

PBS, pH7.2, 0.09% NaN3 and 0.2% (w/v) BSA

description :

Monoclonal clone# G2 antibody for CD45/PTPRC detection. Host: Mouse.Size: 25 Tests, 100 Tests, 200 Tests. Tested applications: Flow Cytometry. Reactive species: Human. CD45/PTPRC information: Molecular Weight: 147254 MW; Subcellular Localization: Membrane ; Single-pass type I membrane protein . Membrane raft . Colocalized with DPP4 in membrane rafts; Tissue Specificity: ; .

short description :

Mouse Monoclonal Anti-human CD45 FITC Conjugated, designed for Flow Cytometry and validated by Flow Cytometry using Human cells.

description1 :

Introduction . The CD45 antigen is present on all human leucocytes including lymphocytes, monocytes, granulocytes, eosinophils, and basophils in peripheral blood. The antigen has a role in signal transduction, modifying signals from other surface molecules. The CD45 antibody has been reported to react weakly with mature circulating erythrocytes and plateletsThe antibody recognizes the human leukocyte common antigen which is found on cells from spleen, lymph nodes, 83% bone marrow cells and granulocytes. The antigen recognized by F10-89-4 is a glycoprotein with a molecular weight of 190kDa to 215kDa. . This antibody is routinely tested by flow cytometric analysis. Flow cytometry and other applications were tested during antibody development by CapricoBio or are reported in the literature. Application Information . Each lot of this antibody has been pre-titrated and tested by flow cytometric analysis of human PBMCs such that 5ul of this product is sufficient for staining of 1 million cells in a 100ul staining volume or 100ul of whole blood. It is recommended that antibody reactivity be empirically titrated for optimal performance in the application of interest.

size :

25 Tests, 100 Tests, 200 Tests

uniprot id :

P08575

host :

Mouse

immunogen :

Human T lymphocytes

form :

Liquid

purification :

Protein A purified

storage :

4 C and protect from prolonged exposure to light. Do not freeze.

isotype :

IgG2a,k

sensitivity :

5ul per sample

application details :

User need to optimize the dilution ratio for this antibody

applications :

Flow Cytometry

reactivity :

Human

image labels :

PBMCs lymphocytes stained with FITC conjugated anti-human CD45 (clone F10- 89-4) (red histogram). PBMCs lymphocytes stained with isotype control IgG1 MOPC- 31C FITC (blue histogram).

background :

CD45(Cluster of Differentiation 45), also known as PTPRC, LCA or CD45R, is an enzyme that, in humans, is encoded by the PTPRC gene.CD45 is a member of the protein tyrosine phosphatase(PTP) family. CD45 is a major high molecular mass leukocyte cell surface molecule which is also an integral membrane protein tyrosine phosphatase.The cytogenetic location of CD45 is 1q31.3-q32.1. CD45 is especially a prototype for transmembrane protein-tyrosine phosphatase(PTP). Targeted disruption of the CD45 gene leads to enhanced cytokine and interferon receptor-mediated activation of JAKs and STAT proteins. In vitro, CD45 directly dephosphorylates and binds to JAKs. Functionally, CD45 negatively regulates interleukin-3-mediated cellular proliferation, erythropoietin-dependent hematopoiesis, and antiviral responses in vitro and in vivo. CD45 has been best studied in T cells, where it determines T cell receptor signaling thresholds. CD45 is moved into or out of the immunological synapse(IS) membrane microdomain depending on the relative influence of interaction with the extracellular galectin lattice or the intracellular actin cytoskeleton. Galectin interaction can be finetuned by varying usage of the heavily Oglycosylated spliced regions and sialylation of Nlinked carbohydrates.

research category :

Adapters, Adaptive Immunity, B Cells, Cell Type Markers, Immunology, Protein Phosphorylation, Signal Transduction, T Cells, Transmembrane, Tyrosine Phosphatases

synonyms :

Receptor-type tyrosine-protein phosphatase C;3.1.3.48;Leukocyte common antigen;L-CA;T200;CD45;PTPRC;CD45;

gene full name :

Receptor-type tyrosine-protein phosphatase C

molecular weight :

147254 MW

protein function :

Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity).

subcellular localization :

Membrane ; Single-pass type I membrane protein . Membrane raft . Colocalized with DPP4 in membrane rafts.

protein name :

Receptor-type tyrosine-protein phosphatase C

last modified :

2/6/19 22:57