Anti-human CD4 Monoclonal Antibody Unconjugated, Flow Validated

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

antibody

product name :

Anti-human CD4 Monoclonal Antibody Unconjugated, Flow Validated

catalog :

FC00344

quantity :

100Âµg, 500Âµg

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

OKT4

reactivity :

human

application :

flow cytometry

product information

sku :

FC00344

status :

Enabled

name :

Anti-human CD4 Monoclonal Antibody Unconjugated, Flow Validated

category name :

Primary Antibodies, Monoclonal Antibodies, Flow Cytometry Antibodies

gene name :

CD4

price various sizes :

100ug / $79 500ug / $320 25 tests / $79

clonality :

Monoclonal

clone number :

OKT4

concentration :

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

conjugate :

contents :

PBS, pH7.2, 0.09% NaN3

description :

Monoclonal clone# G2 antibody for CD4 detection. Host: Mouse.Size: 100Âµg, 500Âµg. Tested applications: Flow Cytometry. Reactive species: Human. CD4 information: Molecular Weight: 51111 MW; Subcellular Localization: Cell membrane ; Single-pass type I membrane protein . Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.

short description :

Mouse Monoclonal Anti-human CD4 Unconjugated, designed for Flow Cytometry and validated by Flow Cytometry using Human cells.

description1 :

Introduction . CD4 is a 55kD type I transmembrane glycoprotein and a member of the immunoglobulin superfamily. It is a specific marker for T lymphocytes, monocytes, macrophages, and dendritic cells. Through interaction of MHC-II, CD4 facilitates cell-cell interaction, thymic differentiation, and activation of downstream signaling cascades. HIV infection of T-cells is instigated through binding of HIV to CD4. The OKT4 antibody binds to the D3 domain of CD4 and does not inhibit HIV binding. . This antibody is routinely tested by flow cytometric analysis. Flow cytometry and other applications were tested during antibody development by CapricoBio or are reported in the literature. Application Information . CD4 is a 55kD type I transmembrane glycoprotein and a member of the immunoglobulin superfamily. It is a specific marker for T lymphocytes, monocytes, macrophages, and dendritic cells. Through interaction of MHC-II, CD4 facilitates cell-cell interaction, thymic differentiation, and activation of downstream signaling cascades. HIV infection of T-cells is instigated through binding of HIV to CD4. The OKT4 antibody binds to the D3 domain of CD4 and does not inhibit HIV binding.

size :

100Âµg, 500Âµg

uniprot id :

P01730

host :

Mouse

immunogen :

Human peripheral blood T lymphocytes

form :

Liquid

purification :

Protein A purified

storage :

4 C . Do not freeze.

isotype :

IgG2b,k

sensitivity :

5ul per sample

application details :

User need to optimize the dilution ratio for this antibody

applications :

Flow Cytometry

reactivity :

Human

image labels :

Lymphocyte gated PBMCs stained with purified anti-human CD4 (clone OKT-4) followed by Goat anti-mouse IgG (H+L) Alexa Fluor 488 (red histogram). Lymphocyte gated PBMCs stained with only Goat anti-mouse IgG (H+L) Alexa Fluor 488 (Blue histogram).

background :

CD stands for "cluster of differentiation"; the number that follows is arbitrarily assigned. In the full designation the cell type and nature and molecular weight of the antigen are given in brackets; for CD4, this is as follows: [T,gp55]. CD4 is present on a subset of T lymphocytes("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.

competitor equivalent skus :

sc 19643 sc 13573 sc 59041 sc 53664 sc 52385 sc 19641 sc 51547 sc 51545 sc 7219 sc 51544 sc 1140 sc 59034 sc 32811 sc 70659 sc 70670 sc 70674 sc 59032 sc 1176 sc 59031 sc 70660 sc 59042 sc 53838 sc 20079 sc 53042 sc 53041 sc 19642 sc 514746 sc 514571

research category :

Adaptive Immunity, Antiviral Signaling, Hematopoietic Progenitors, Immune System Diseases, Immunology, Lymphoid, Myeloid, Stem Cells, T Cells, T Lymphocytic Lineage

synonyms :

T-cell surface glycoprotein CD4;T-cell surface antigen T4/Leu-3;CD4;CD4;

gene full name :

T-cell surface glycoprotein CD4

molecular weight :

51111 MW

protein function :

Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts.

subcellular localization :

Cell membrane ; Single-pass type I membrane protein . Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum.

protein name :

T-cell surface glycoprotein CD4

last modified :

2/6/19 22:57