Anti-human CD11b Itgam Monoclonal Antibody PerCP Conjugated, Flow Validated

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product summary

company name :

Boster

product type :

antibody

product name :

Anti-human CD11b Itgam Monoclonal Antibody PerCP Conjugated, Flow Validated

catalog :

FC00144-PerCP

quantity :

100 Tests, 200 Tests

clonality :

monoclonal

host :

mouse

conjugate :

PerCP

clone name :

OKM1

The same clone is also sold as:

Caprico Biotechnologies: 100765, 100701, 100715, 100745, 100735, 100725

reactivity :

human, mouse

application :

flow cytometry

product information

SKU :

FC00144-PerCP

Product Name :

Anti-human CD11b Itgam Monoclonal Antibody PerCP Conjugated, Flow Validated

Size :

100 Tests, 200 Tests

Clonality :

Monoclonal

Clone Number :

OKM1

Host :

Mouse

Conjugate :

PerCP

Reactivity :

Human

Application(s) :

Flow Cytometry

Application Details :

User need to optimize the dilution ratio for this antibody

Description :

Boster Bio Anti-human CD11b Itgam Monoclonal Antibody PerCP Conjugated, Flow Validated catalog # FC00144-PerCP. Tested in Flow Cytometry applications. This antibody reacts with Human. Introduction: CD11b is a 165-kDa adhesion glycoprotein that associates with the 95-kDa integrin β2 (CD18) to form the CD11b/CD18 complex, also known as Mac-1 or CR3. CD11b is expressed on activated lymphocytes, monocytes, granulocytes, and a subset of NK cells. CD11b functions in cell-cell and cell-substrate interactions and is a receptor for iC3b, CD54 (ICAM-1), CD102 (ICAM-2) and CD50 (ICAM-3).The OKM1 antibody directed against the CD11b antigen on human monocytes and granulocytes. This antibody is routinely tested by flow cytometric analysis. Flow cytometry and other applications were tested during antibody development by Boster or are reported in the literature. Application Information: Each lot of this antibody has been pre-titrated and tested by flow cytometric analysis of human PBMCs such that 5µl of this product is sufficient for staining of 1 million cells in a 100µl staining volume or 100µl of whole blood. It is recommended that antibody reactivity be empirically titrated for optimal performance in the application of interest.

Concentration :

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Gene Name :

Itgam

Uniprot ID :

P05555

Immunogen :

Human lymph node lymphocytes

Form :

Liquid

Contents :

PBS, pH7.2, 0.09% NaN3 and 0.2% (w/v) BSA

Purification :

Protein A purified

Isotype :

IgG2b,k

Storage :

4°C and protect from prolonged exposure to light. Do not freeze.

Gene Full Name :

Integrin alpha-M

Synonyms :

Integrin alpha-M; CD11 antigen-like family member B; CR-3 alpha chain; Cell surface glycoprotein MAC-1 subunit alpha; Leukocyte adhesion receptor MO1; CD11b; Itgam;

Protein Name :

Integrin alpha-M

Molecular Weight :

127481 MW

Protein Function :

Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Alpha-M/beta-2 play a critical role in mast cell development and in immune complex-mediated glomerulonephritis. Mice expressing a null mutation of the alpha-M subunit gene demonstrate increase in neutrophil accumulation, in response to a impaired degranulation and phagocytosis, events that apparently accelerate apoptosis in neutrophils. These mice develop obesity.

Subcellular Localization :

Membrane; Single-pass type I membrane protein.

Tissue Specificity :

Predominantly expressed in monocytes and granulocytes.

Sequence Similarities :

Belongs to the integrin alpha chain family.

Background :

ITGAM (Integrin Alpha-M), also called CD11B or Mo1 ALPHA SUBUNIT (MO1A), is one protein subunit that forms the heterodimeric integrin alpha-M beta-2 (alphaMbeta2) molecule. A major surface antigen family on human leukocytes includes complement receptor type 3 (CR3A; also called ITGAM, Mac1, or Mo1), lymphocyte function-associated (LFA) antigen type 1 (ITGAL), and p150, 95 (ITGAX). By in situ hybridization, Corbi et al. (1988) demonstrated that the genes encoding the alpha subunits of LFA1 (ITGAL), Mac1, and p150, 95 (ITGAX), all of which are involved in leukocyte adhesion, constitute a cluster on 16p13.1-p11. Callen et al. (1991) narrowed the assignment to 16p11.2. Inflammation plays an essential role in the initiation and progression of atherosclerosis. Simon et al. (2000) presented evidence that it also has a role in vascular repair after mechanical arterial injury (i.e., percutaneous transluminal coronary angioplasty, or PTCA).

Research Category :

Cell Adhesion, Cell Type Marker, Cell Type Markers, Cytoskeleton / Ecm, Hematopoietic Progenitors, Integrins, Lymphoid, Mesenchymal Stem Cells, Myeloid, Neuroscience, Signal Transduction, Stem Cells, T Lymphocytic Lineage