Anti-human CD14 Monoclonal Antibody Unconjugated, Flow Validated

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product summary

company name :

Boster

product type :

antibody

product name :

Anti-human CD14 Monoclonal Antibody Unconjugated, Flow Validated

catalog :

FC00137

quantity :

100 ug, 500 ug

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

26ic

reactivity :

human

application :

flow cytometry

product information

SKU :

FC00137

Product Name :

Anti-human CD14 Monoclonal Antibody Unconjugated, Flow Validated

Size :

100 ug, 500 ug

Clonality :

Monoclonal

Clone Number :

26ic

Host :

Mouse

Reactivity :

Human

Application(s) :

Flow Cytometry

Application Details :

User need to optimize the dilution ratio for this antibody

Description :

Boster Bio Anti-human CD14 Monoclonal Antibody Unconjugated, Flow Validated catalog # FC00137. Tested in Flow Cytometry applications. This antibody reacts with Human. Introduction: Clone 26ic reacts with a nonfunctional domain of human CD14, a 53-55 kDa glycosylphosphatidylinositol (GPI)-anchored and single chain glycoprotein expressed at high levels on monocytes. Additionally, CD14 antibody reacts with interfollicular macrophages, reticular dendritic cells and some Langerhans cells. The binding of CD14 antibody does not inhibit CD14 mediated activities, and is useful for detecting CD14 expression by immunofluorescence and or immunocytochemical methods. This antibody is routinely tested by flow cytometric analysis. Flow cytometry and other applications were tested during antibody development by Boster or are reported in the literature. Application Information: Each lot of this antibody has been quality control tested by flow cytometric analysis of human PBMCs. For flow cytometric staining, the recommended use of this antibody is 0.5µg per 1x106 cells in 100µl of staining volume followed by any fluorescent conjugated anti-mouse secondary antibody. It is strongly suggested that the antibody reactivity be empirically titrated for optimal performance in the application of interest.

Concentration :

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Gene Name :

CD14

Uniprot ID :

P08571

Immunogen :

Cultured human peripheral blood monocytes

Form :

Liquid

Contents :

PBS, pH7.2, 0.09% NaN3

Purification :

Protein A purified

Isotype :

IgG2b,k

Storage :

Store at 4°C for one year. Do not freeze.

Gene Full Name :

Monocyte differentiation antigen CD14

Synonyms :

Monocyte differentiation antigen CD14 ; Myeloid cell-specific leucine-rich glycoprotein; CD14; Monocyte differentiation antigen CD14, urinary form; Monocyte differentiation antigen CD14, membrane-bound form; CD14;

Protein Name :

Monocyte differentiation antigen CD14

Molecular Weight :

40076 MW

Protein Function :

Coreceptor for bacterial lipopolysaccharide (PubMed:1698311, PubMed:23264655). In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS) (PubMed:20133493, PubMed:23264655). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed:8612135). Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway (PubMed:16880211). Binds electronegative LDL (LDL (-)) and mediates the cytokine release induced by LDL (-) (PubMed:23880187).

Subcellular Localization :

Cell membrane ; Lipid- anchor, GPI-anchor. Secreted. Membrane raft. Golgi apparatus. Secreted forms may arise by cleavage of the GPI anchor.

Tissue Specificity :

Detected on macrophages (at protein level) (PubMed:1698311). Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.

Background :

CD14, Cluster of differentiation 14, ingle-copy gene encoding 2 protein forms: a 50- to 55-kD glycosylphosphatidylinositol-anchored membrane protein (mCD14) and a monocyte or liver-derived soluble serum protein (sCD14) that lacks the anchor. By in situ hybridization and study of somatic cell hybrid DNA that the gene is located at bands 5q23-q31. CD14 acts as a co-receptor (along with the Toll-like receptor TLR 4 and MD-2) for the detection of bacterial lipopolysaccharide (LPS). CD14 can bind LPS only in the presence of lipopolysaccharide-binding protein (LBP). Although LPS is considered its main ligand, CD14 also recognizes other pathogen-associated molecular patterns.

Research Category :

Cell Type Markers, Hematopoietic Progenitors, Immunology, Innate Immunity, Mesenchymal Stem Cells, Myeloid, Stem Cells, Tlr Signaling