Human CD32/FCGR2b/c ELISA Kit EZ-Set™ (DIY Antibody Pairs)

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human CD32/FCGR2b/c ELISA Kit EZ-Set™ (DIY Antibody Pairs)

catalog :

EZ1312

quantity :

For 5 plates, 96 wells each

product information

SKU :

EZ1312

Product Name :

Human CD32/FCGR2b/c ELISA Kit EZ-Set™ (DIY Antibody Pairs)

Size :

For 5 plates, 96 wells each

Description :

Human CD32/FCGR2b/c ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human FCGR2B in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml. Product Features: Your ideal low cost ELISA solution. EZ Set™ ELISA kit contains the necessary core reagents develop and run the ELISA, including ELISA-optimized matched antibody pairs, recombinant protein standards, and HRP conjugated Avidin-Biotin-Complex (ABC). One kit is sufficient for developing 5 plates (96 well format). EZ Set™ ELISAs are ideal for experienced ELISA users on a budget and great for exploratory work--a fast and easy way to identify the relevant protein targets of interest/relevance to your studies.

Reactivity :

Human

Application :

ELISA

Gene Name :

FCGR2B

Uniprot ID :

P31994&P31995

Immunogen :

Expression system for standard: NS0; Immunogen sequence: A46-P217

Cross-reactivity :

There is no detectable cross-reactivity with other relevant proteins.

Assay Range :

46.9 pg/ml - 3,000 pg/ml

Sensitivity :

<10 pg/ml

Sample Type :

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Gene Full Name :

Fc fragment of IgG, low affinity IIb, receptor (CD32)

Synonyms :

Low affinity immunoglobulin gamma Fc region receptor II-b; IgG Fc receptor II-b; CDw32; Fc-gamma RII-b; Fc-gamma-RIIb; FcRII-b; CD32; FCGR2B; CD32, FCG2, IGFR2

Protein Function :

Receptor for the Fc region of complexed or aggregated immunoglobulins gamma. Low affinity receptor. Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B- cells. Binding to this receptor results in down-modulation of previous state of cell activation triggered via antigen receptors on B-cells (BCR), T-cells (TCR) or via another Fc receptor. Isoform IIB1 fails to mediate endocytosis or phagocytosis. Isoform IIB2 does not trigger phagocytosis.

Subcellular Localization :

Cell membrane; Single-pass type I membrane protein.

Tissue Specificity :

Is the most broadly distributed Fc-gamma- receptor. Expressed in monocyte, neutrophils, macrophages, basophils, eosinophils, Langerhans cells, B-cells, platelets cells and placenta (endothelial cells). Not detected in natural killer cells.

Background :

CD32 is a surface receptor protein and part of a large population of B cell co-receptors, which act to modulate signaling. It has a low-affinity for IgG antibodies and down-regulates antibody production in the presence of IgG. This feedback loops essentially lowers the production of IgG produced by B cells when there is a surplus in the body.

Research Category :

Adaptive Immunity, B Cells, Hematopoietic Progenitors, Immunoglobulins, Immunology, Innate Immunity, Lymphoid, Macrophage / Inflamm., Myeloid, Receptors, Stem Cells, T Lymphocytic Lineage