Human FGF21 ELISA Kit EZ-Set™ (DIY Antibody Pairs) | Boster EZ0994 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human FGF21 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

catalog :

EZ0994

quantity :

For 5 plates, 96 wells each

price :

215 USD

more info or order :

Boster product webpage

citations: 3

Reference
Gallego Escuredo J, Gomez Ambrosi J, Catalan V, Domingo P, Giralt M, Fruhbeck G, et al. Opposite alterations in FGF21 and FGF19 levels and disturbed expression of the receptor machinery for endocrine FGFs in obese patients. Int J Obes (Lond). 2015;39:121-9 pubmed publisher
Hindricks J, Ebert T, Bachmann A, Kralisch S, Lossner U, Kratzsch J, et al. Serum levels of fibroblast growth factor-21 are increased in chronic and acute renal dysfunction. Clin Endocrinol (Oxf). 2014;80:918-24 pubmed publisher
Wang D, Zhu W, Li J, An C, Wang Z. Serum concentrations of fibroblast growth factors 19 and 21 in women with gestational diabetes mellitus: association with insulin resistance, adiponectin, and polycystic ovary syndrome history. PLoS ONE. 2013;8:e81190 pubmed publisher

image

image 1 :

Human FGF21 EZ Set ELISA Kit standard curve

product information

SKU :

EZ0994

Product Name :

Human FGF21 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

Size :

For 5 plates, 96 wells each

Price :

215 USD

Description :

Human FGF21 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human FGF21 in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml. Product Features: Your ideal low cost ELISA solution. EZ Set™ ELISA kit contains the necessary core reagents develop and run the ELISA, including ELISA-optimized matched antibody pairs, recombinant protein standards, and HRP conjugated Avidin-Biotin-Complex (ABC). One kit is sufficient for developing 5 plates (96 well format). EZ Set™ ELISAs are ideal for experienced ELISA users on a budget and great for exploratory work--a fast and easy way to identify the relevant protein targets of interest/relevance to your studies.

Reactivity :

Human

Application :

ELISA

Gene Name :

FGF21

Uniprot ID :

Q9NSA1

Immunogen :

Expression system for standard: E.coli; Immunogen sequence: H29-S209

Cross-reactivity :

There is no detectable cross-reactivity with other relevant proteins.

Assay Range :

31.2 pg/ml - 2,000 pg/ml

Sensitivity :

<10 pg/ml

Sample Type :

cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA)

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Gene Full Name :

Fibroblast growth factor 21

Synonyms :

Fibroblast growth factor 21;FGF-21;FGF21;UNQ3115/PRO10196;

Protein Name :

Fibroblast growth factor 21

Molecular Weight :

22300 MW

Protein Function :

Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB.

Subcellular Localization :

Secreted .

Sequence Similarities :

Belongs to the heparin-binding growth factors family.

Background :

Fibroblast growth factor 21 is a protein that in humans is encoded by the FGF21 gene. By genomic sequence analysis, Nishimura et al. (2000) identified the FGF21 gene within the 5-prime flanking region of the FUT1 gene on chromosome 19. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF21 stimulates glucose uptake in adipocytes but not in other cell types. This effect is additive to the activity of insulin. FGF21 treatment of adipocytes is associated with phosphorylation of FRS2, a protein linking FGF receptors to the Ras/MAP kinase pathway. FGF21 injection in ob/ob mice results in an increase in Glut1 in adipose tissue. FGF21 also protects animals from diet-induced obesity when overexpressed in transgenic mice and lowers blood glucose and triglyceride levels when administered to diabetic rodents.

Research Category :

Angiogenesis, Cancer, Cardiovascular, Growth Factors, Growth Factors/Hormones, Signal Transduction

more info or order :

Boster product webpage