ELISA/assay

Human IL-33 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

EZ0929

For 5 plates, 96 wells each

Human IL-33 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

For 5 plates, 96 wells each

Human IL-33 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human IL33 in cell culture supernatants, serum, plasma (heparin, EDTA) and cell lysates. Sensitivity: 10 pg/ml. Product Features: Your ideal low cost ELISA solution. EZ Set™ ELISA kit contains the necessary core reagents develop and run the ELISA, including ELISA-optimized matched antibody pairs, recombinant protein standards, and HRP conjugated Avidin-Biotin-Complex (ABC). One kit is sufficient for developing 5 plates (96 well format). EZ Set™ ELISAs are ideal for experienced ELISA users on a budget and great for exploratory work--a fast and easy way to identify the relevant protein targets of interest/relevance to your studies.

Human

ELISA

IL33

O95760

Expression system for standard: E.coli; Immunogen sequence: S112-T270

There is no detectable cross-reactivity with other relevant proteins.

15.6 pg/ml - 1,000 pg/ml

<10 pg/ml

cell culture supernatants, serum, plasma (heparin, EDTA) and cell lysate

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

interleukin 33

C9orf26; DVS27; DVS27 related protein; IL-1F11; IL-33; IL1F11; IL33; IL33_HUMAN; Interleukin-1 family member 11; Interleukin-33; NF-HEV; NFEHEV; Nuclear factor from high endothelial venules; RP11-575C20.2;

Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells. Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as a chemoattractant for Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury. In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB/RELA, lowering expression of its targets. This form is rapidely lost upon angiogenic or proinflammatory activation.

Secreted, Nucleus, Chromosome, secretory vesicle

Expressed at high level in high endothelial venules found in tonsils, Peyer patches and mesenteric lymph nodes. Almost undetectable in placenta.

Interleukin 33 (IL-33) is a cytokine belonging to the IL-1 superfamily. The IL33 gene maps to chromosome 9p24.1 by using of genomic database analysis. Recombinant mature human IL33 bound to ST2. The induction of type 2 cytokines by IL-33 in vivo is believed to induce the severe pathological changes observed in mucosal organs following administration of IL-33. IL33, an alarmin released from necrotic cells, is necessary for potent CD8 + T cell (CTL) responses to replicating, prototypic RNA, and DNA viruses in mice. IL33 prevented the downregulation of CXCR2 and inhibition of chemotaxis induced by activation of TLR4, and found that IL33 reverses the TLR4-induced reduction of CXCR2 expression via the inhibition of expression of GRK2.

Angiogenesis, Cardiovascular, Cytokines, Hypertrophy, Immunology, Innate Immunity, Interleukins