Human Tryptase/TPSAB1,B2 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human Tryptase/TPSAB1,B2 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

catalog :

EZ0898

quantity :

For 5 plates, 96 wells each

product information

SKU :

EZ0898

Product Name :

Human Tryptase/TPSAB1,B2 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

Size :

For 5 plates, 96 wells each

Description :

Human Tryptase/TPSAB1,B2 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human TPSB2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 15 pg/ml. Product Features: Your ideal low cost ELISA solution. EZ Set™ ELISA kit contains the necessary core reagents develop and run the ELISA, including ELISA-optimized matched antibody pairs, recombinant protein standards, and HRP conjugated Avidin-Biotin-Complex (ABC). One kit is sufficient for developing 5 plates (96 well format). EZ Set™ ELISAs are ideal for experienced ELISA users on a budget and great for exploratory work--a fast and easy way to identify the relevant protein targets of interest/relevance to your studies.

Reactivity :

Human

Application :

ELISA

Gene Name :

TPSB2

Uniprot ID :

P20231

Immunogen :

Expression system for standard: NS0; Immunogen sequence: M1-V275

Cross-reactivity :

There is no detectable cross-reactivity with other relevant proteins.

Assay Range :

156 pg/ml - 10,000 pg/ml

Sensitivity :

<15 pg/ml

Sample Type :

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Gene Full Name :

Tryptase beta-2

Synonyms :

Tryptase beta-2;Tryptase-2;3.4.21.59;Tryptase II;TPSB2;TPS2;

Protein Name :

Tryptase alpha/beta-1/beta-2

Molecular Weight :

30515 MW

Protein Function :

Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. May play a role in innate immunity.

Subcellular Localization :

Secreted. Released from the secretory granules upon mast cell activation.

Tissue Specificity :

TPSAB1: Isoform 1 and isoform 2 are expressed in lung,stomach, spleen, heart and skin; in these tissues, isoform 1 ispredominant. Isoform 2 is expressed in aorta, spleen, and breasttumor, with highest levels in the endothelial cells of some bloodvessels surrounding the aorta, as well as those surrounding thetumor and low levels, if any, in mast cells (at protein level).

Background :

Tryptases are serine proteases implicated in asthma and are highly expressed in human mast cells. They are derived from at least 4 nonallelic genes clustered on chromosome 16p13.3: TPSAB1, which represents the alpha and beta-I tryptase alleles; TPSB2, which represents the beta-II and beta-III tryptase alleles; TPSG1; and TPSD1. Elevated levels of serum tryptase occur in both anaphylactic and anaphylactoid reactions, but a negative test does not exclude anaphylaxis. Tryptase is less likely to be elevated in food allergy reactions as opposed to other causes of anaphylaxis.

Research Category :

Cell Biology, Immunology, Innate Immunity, Interspecies Interaction, Microbiology, Proteolysis / Ubiquitin, Proteolytic Enzymes, Secreted Molecules