Mouse CXCL4/PF4 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

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product summary

company name :

Boster

product type :

ELISA/assay

product name :

Mouse CXCL4/PF4 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

catalog :

EZ0727

quantity :

For 5 plates, 96 wells each

product information

SKU :

EZ0727

Product Name :

Mouse CXCL4/PF4 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

Size :

For 5 plates, 96 wells each

Description :

Mouse CXCL4/PF4 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Mouse Pf4 in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA). Sensitivity: 1 pg/ml. Product Features: Your ideal low cost ELISA solution. EZ Set™ ELISA kit contains the necessary core reagents develop and run the ELISA, including ELISA-optimized matched antibody pairs, recombinant protein standards, and HRP conjugated Avidin-Biotin-Complex (ABC). One kit is sufficient for developing 5 plates (96 well format). EZ Set™ ELISAs are ideal for experienced ELISA users on a budget and great for exploratory work--a fast and easy way to identify the relevant protein targets of interest/relevance to your studies.

Reactivity :

Mouse

Application :

ELISA

Gene Name :

Pf4

Uniprot ID :

Q9Z126

Immunogen :

Expression system for standard: E.coli; Immunogen sequence: V30-S105

Cross-reactivity :

There is no detectable cross-reactivity with other relevant proteins.

Assay Range :

15.6 pg/ml - 1,000 pg/ml

Sensitivity :

<1 pg/ml

Sample Type :

cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA)

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Gene Full Name :

Platelet factor 4

Synonyms :

Platelet factor 4;PF-4;C-X-C motif chemokine 4;Pf4;Cxcl4, Scyb4;

Protein Name :

Platelet factor 4

Molecular Weight :

11243 MW

Protein Function :

Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation (By similarity).

Subcellular Localization :

Secreted.

Background :

Platelet factor 4 (PF4) is a small cytokine belonging to the CXC chemokine family that is also known as chemokine (C-X-C motif) ligand 4 (CXCL4). The PF4 gene was localized on map to 4q12-q13. Chemokines play fundamental roles in the development, homeostasis, and function of the immune system, and they have effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis. Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair.

Research Category :

Atherosclerosis, Cardiovascular, Chemokines, Immunology, Innate Immunity, Macrophage / Inflamm., Platelets, Vascular Inflammation