Human Cystatin C ELISA Kit EZ-Set™ (DIY Antibody Pairs)

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product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human Cystatin C ELISA Kit EZ-Set™ (DIY Antibody Pairs)

catalog :

EZ0678

quantity :

For 5 plates, 96 wells each

product information

SKU :

EZ0678

Product Name :

Human Cystatin C ELISA Kit EZ-Set™ (DIY Antibody Pairs)

Size :

For 5 plates, 96 wells each

Description :

Human Cystatin C ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human CST3 in cell culture supernatants, serum, plasma (heparin, EDTA), saliva, urine and human milk. Sensitivity: 10 pg/ml. Product Features: Your ideal low cost ELISA solution. EZ Set™ ELISA kit contains the necessary core reagents develop and run the ELISA, including ELISA-optimized matched antibody pairs, recombinant protein standards, and HRP conjugated Avidin-Biotin-Complex (ABC). One kit is sufficient for developing 5 plates (96 well format). EZ Set™ ELISAs are ideal for experienced ELISA users on a budget and great for exploratory work--a fast and easy way to identify the relevant protein targets of interest/relevance to your studies.

Reactivity :

Human

Application :

ELISA

Gene Name :

CST3

Uniprot ID :

P01034

Immunogen :

Expression system for standard: NS0; Immunogen sequence: M1-A146

Cross-reactivity :

There is no detectable cross-reactivity with other relevant proteins.

Assay Range :

312 pg/ml - 20,000 pg/ml

Sensitivity :

<10 pg/ml

Sample Type :

cell culture supernatants, serum, plasma (heparin, EDTA), saliva, urine and human milk

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Gene Full Name :

cystatin C

Synonyms :

ARMD11; bA218C14.4 (cystatin C); Cst3; Cystatin-3; Cystatin-C; CYTC_HUMAN; Gamma-trace; Neuroendocrine basic polypeptide; Post-gamma-globulin;

Protein Function :

As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity.

Subcellular Localization :

Secreted

Tissue Specificity :

Expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). Expressed in various body fluids, such as the cerebrospinal fluid and plasma. Expressed in highest levels in the epididymis, vas deferens, brain, thymus, and ovary and the lowest in the submandibular gland.

Background :

Cystatin C or cystatin 3 (formerly gamma trace, post-gamma-globulin or neuroendocrine basic polypeptide), a protein encoded by the CST3 gene, was originally described as a constituent of normal cerebrospinal fluid (CSF) and of urine from patients with renal failure.1 Cystatin 3 has a low molecular weight (approximately 13.3 kilodaltons), and it is removed from the bloodstream by glomerular filtration in the kidneys. In humans, all cells with a nucleus (cell core containing the DNA) produce cystatin C as a chain of 120 amino acids. It is found in virtually all tissues and bodily fluids. Cystatin C, which belongs to the type II cystatin gene family, is a potent inhibitor of lysosomal proteinases2 (enzymes from a special subunit of the cell that break down proteins) and probably one of the most important extracellular inhibitors of cysteine proteases3 (it prevents the breakdown of proteins outside the cell by a specific type of protein degrading enzymes). Moreover, cystatin C is involved in network reorganization in the epileptic dentate gyrus.4

Research Category :

Apoptosis, Atherosclerosis, Cardiovascular, Caspases Etc, Cell Biology, Cell Type Markers, Intracellular, Neural Stem Cells, Stem Cells, Tags & Cell Markers, Tumor Associated