ELISA/assay

Human Angiopoietin-2 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

EZ0657

For 5 plates, 96 wells each

Human Angiopoietin-2 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

For 5 plates, 96 wells each

Human Angiopoietin-2 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human ANGPT2 in cell culture supernatants, serum, plasma (heparin, EDTA) , saliva and human milk. Sensitivity: 10 pg/ml. Product Features: Your ideal low cost ELISA solution. EZ Set™ ELISA kit contains the necessary core reagents develop and run the ELISA, including ELISA-optimized matched antibody pairs, recombinant protein standards, and HRP conjugated Avidin-Biotin-Complex (ABC). One kit is sufficient for developing 5 plates (96 well format). EZ Set™ ELISAs are ideal for experienced ELISA users on a budget and great for exploratory work--a fast and easy way to identify the relevant protein targets of interest/relevance to your studies.

Human

ELISA

ANGPT2

O15123

Expression system for standard: NS0; Immunogen sequence: Y19-F496

There is no detectable cross-reactivity with other relevant proteins.

156 pg/ml - 10,000 pg/ml

<10 pg/ml

cell culture supernatants, serum, plasma (heparin, EDTA), saliva and human milk

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

angiopoietin 2

Angiopoietin-2; ANG-2; ANGPT2;

Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal.

Secreted

Angiopoietin-2 is a protein that in humans is encoded by the ANGPT2 gene. Angiopoietins are members of the vascular endothelial growth factor family and the only known growth factors largely specific for vascular endothelium. ANGPT2 is a naturally occurring antagonist of angiopoietin-1 that competes for binding to the TIE2 receptor and blocks ANGPT1-induced TIE2 autophosphorylation during vasculogenesis. Its expression occurs at sites of vascular remodelling: dorsal aorta and major aortic branches, ovary, placenta and uterus.

Angiogenesis, Cardiovascular, Growth Factors