Human Trail ELISA Kit EZ-Set™ (DIY Antibody Pairs) | Boster EZ0532 product information

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product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human Trail ELISA Kit EZ-Set™ (DIY Antibody Pairs)

catalog :

EZ0532

quantity :

For 5 plates, 96 wells each

product information

SKU :

EZ0532

Product Name :

Human Trail ELISA Kit EZ-Set™ (DIY Antibody Pairs)

Size :

For 5 plates, 96 wells each

Description :

Human Trail ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human TNFSF10 in cell culture supernatants, cell lysates, serum, plasma (heparin, EDTA) and saliva. Sensitivity: 1 pg/ml. Product Features: Your ideal low cost ELISA solution. EZ Set™ ELISA kit contains the necessary core reagents develop and run the ELISA, including ELISA-optimized matched antibody pairs, recombinant protein standards, and HRP conjugated Avidin-Biotin-Complex (ABC). One kit is sufficient for developing 5 plates (96 well format). EZ Set™ ELISAs are ideal for experienced ELISA users on a budget and great for exploratory work--a fast and easy way to identify the relevant protein targets of interest/relevance to your studies.

Reactivity :

Human

Application :

ELISA

Gene Name :

TNFSF10

Uniprot ID :

P50591

Immunogen :

Expression system for standard: NS0; Immunogen sequence: T95-G281

Cross-reactivity :

There is no detectable cross-reactivity with other relevant proteins.

Assay Range :

15.6 pg/ml - 1,000 pg/ml

Sensitivity :

<1 pg/ml

Sample Type :

cell culture supernatants, cell lysates, serum, plasma (heparin, EDTA) and saliva

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Gene Full Name :

tumor necrosis factor (ligand) superfamily, member 10

Synonyms :

Tumor necrosis factor ligand superfamily member 10; Apo-2 ligand; Apo-2L; TNF-related apoptosis-inducing ligand; Protein TRAIL; CD253; TNFSF10; APO2L; TRAIL;

Protein Function :

Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis.

Subcellular Localization :

Cell membrane, Single-pass type II membrane protein, Secreted

Tissue Specificity :

Widespread; most predominant in spleen, lung and prostate.

Background :

In the field of cell biology, TNF-related apoptosis-inducing ligand (TRAIL), is a protein which functions as a ligand which induces the process of cell death called apoptosis. TRAIL has also been designated CD253 (cluster of differentiation 253).Tumor necrosis factor (TNF) family cytokines function as prominent mediators of immune regulation and the inflammatory response. Most TNF family cytokines are expressed as type II transmembrane proteins, with homology confined to approximately 150 C-terminal residues. The TNF ligands interact with a parallel family of receptors. TRAIL binds to the death receptors DR4 (TRAIL-RI) and DR5 (TRAIL-RII). The process of apoptosis is caspase-8-dependent. Caspase-8 activates downstream effector caspases including procaspase-3, -6, and -7, leading to activation of specific kinases. TRAIL also binds the receptors DcR1 and DcR2, which do not contain a cytoplasmic domain (DcR1) or contain a truncated death domain (DcR2). The standard product used in this kit is recombinant human TRAIL with the molecular mass of 21KDa. It is expressed from the amino acids ectoderm T95--G28.

Research Category :

Apoptosis, Atherosclerosis, Cancer, Cardiovascular, Cell Biology, Cell Death, Invasion/Microenvironment, Receptors