ELISA/assay

Human Rantes EZ-Set ELISA Kit (DIY Antibody Pairs)

EZ0494

For 5 plates, 96 wells each

Enabled

Human Rantes EZ-Set ELISA Kit (DIY Antibody Pairs)

Picokine ELISA Kits, EZ Set ELISA Kits (Ab Pairs)

CCL5

15.6pg/ml-1000pg/ml

Components Included In The EZ Set Accessory Kit Antibody diluent buffer ABC diluent buffer Sample diluent buffer TMB TMB stop solution 5x 96 well plates, plate sealers . *The EZ Set Accessory Kit provides an easy way to get all the reagents you need for performing an ELISA assay. The components included in the EZ Set Accessory Kit can also be substituted with products other companies if it is of convenience to you. Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

No

A ELISA kit containing the core components for developing solid phase sandwich ELISA assay to quantitatively detects Rantes in Human samples. The kit contains antibody pairs, recombinant protein standards and HRP conjugated ABC.

A ELISA kit containing the core components for developing solid phase sandwich ELISA assay to quantitatively detects Rantes in Human samples. The kit contains antibody pairs, recombinant protein standards and HRP conjugated ABC.

Product Features: . Your ideal low cost ELISA solution. EZ Set ELISA kit contains the necessary core reagents develop and run the ELISA, including ELISA-optimized matched antibody pairs, recombinant protein standards, and HRP conjugated Avidin-Biotin-Complex (ABC). One kit is sufficient for developing 5 plates (96 well format). EZ Set ELISAs are ideal for experienced ELISA users on a budget and great for exploratory work--a fast and easy way to identify the relevant protein targets of interest/relevance to your studies. . Boster also provides the most comprehensive technical support materials and most user friendly technical support service to guarantee that you can do the experiment smoothly and successfully.

For 5 plates, 96 wells each

P13501

No

Expression system for standard: E.coli, S24-S91

Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

There is no detectable cross-reactivity with other relevant proteins.

<3pg/ml

Catalog number Description Quantity Buffers EZ0494-CA Rabbit anti- human Rantes polyclonal antibody(Capture Antibody) 500 uL, 0.4mg/mL(recommended dilution 1:100) 0.5% Na2HPO4, 0.02% Sodium azide and 50% Glycerol EZ0494-DA Biotinylated goat anti- human Rantes polyclonal antibody(Detection Antibody) 500 uL, 12.5ug/mL(recommended dilution 1:100) 0.02% Sodium azide, 0.5%BSA, 0.2%Tris, 1%NaCl and 30% Glycerol EZ0494-ST Lyophilized recombinant human Rantes standard 10ng/tubex2 AR1103 Avidin-Biotin-Peroxidase Complex(ABC) 500ul(recommended dilution 1:100)

cell culture supernates, serum, plasma (heparin, EDTA, citrate) and urine.

ELISA

Human

Human Rantes EZ-Set ELISA Kit standard curve

heparin, EDTA or citrate

1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml, 15.6pg/ml

Rantes(regulated on activation normal T cell expressed and secreted) is one of the natural ligands for the chemokine receptor CCR5 and potently suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Peripheral blood mononuclear cells or CD4(+) lymphocytes obtained from different individuals have wide variations in their ability to secrete RANTES. The Rantes gene product is predicted to be 10 kDa and, after cleavage of the signal peptide, approximately 8 kDa. Of the 68 residues, 4 are cysteines, and there are no sites for N-linked glycosylation. Rantes is expressed by cultured T cell lines that are Ag specific and growth factor dependent.

Antiviral Signaling, Atherosclerosis, Cardiovascular, Chemokines, Immune System Diseases, Immunology, Innate Immunity, Vascular Inflammation

C-C motif chemokine 5; EoCP; Eosinophil chemotactic cytokine; SIS-delta; Small-inducible cytokine A5; T cell-specific protein P228; TCP228; T-cell-specific protein RANTES; RANTES(3-68); RANTES(4-68); CCL5; D17S136E; SCYA5;

chemokine (C-C motif) ligand 5

Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. May activate several chemokine receptors including CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1-68) and RANTES(3-68) and is generated by an unidentified enzyme associated with monocytes and neutrophils. May also be an agonist of the G protein-coupled receptor GPR75, stimulating inositol trisphosphate production and calcium mobilization through its activation. Together with GPR75, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. By activating GPR75 may also play a role in insulin secretion by islet cells.

Secreted

Expressed in the follicular fluid (at protein level). T-cell and macrophage specific.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 35 166 442 31 169 476 Standard deviation 1.71 11.45 22.54 1.58 12.67 33.32 CV(%) 4.9% 6.9% 5.1% 5.1% 7.5% 7%

Concentration(pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.007 0.051 0.099 0.207 0.450 0.934 1.769 2.497

15-20min

5/23/19 3:37