Mouse MMP-9 ELISA Kit EZ-Set™ (DIY Antibody Pairs) | Boster EZ0466 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Mouse MMP-9 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

catalog :

EZ0466

quantity :

For 5 plates, 96 wells each

price :

215 USD

more info or order :

Boster product webpage

citations: 1

Reference
Lerman O, Galiano R, Armour M, Levine J, Gurtner G. Cellular dysfunction in the diabetic fibroblast: impairment in migration, vascular endothelial growth factor production, and response to hypoxia. Am J Pathol. 2003;162:303-12 pubmed

image

image 1 :

Mouse MMP-9 EZ Set ELISA Kit standard curve

product information

SKU :

EZ0466

Product Name :

Mouse MMP-9 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

Size :

For 5 plates, 96 wells each

Price :

215 USD

Description :

Mouse MMP-9 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Mouse Mmp9 in cell culture supernatants, serum and plasma ( heparin). Sensitivity: 20 pg/ml. Product Features: Your ideal low cost ELISA solution. EZ Set™ ELISA kit contains the necessary core reagents develop and run the ELISA, including ELISA-optimized matched antibody pairs, recombinant protein standards, and HRP conjugated Avidin-Biotin-Complex (ABC). One kit is sufficient for developing 5 plates (96 well format). EZ Set™ ELISAs are ideal for experienced ELISA users on a budget and great for exploratory work--a fast and easy way to identify the relevant protein targets of interest/relevance to your studies.

Reactivity :

Mouse

Application :

ELISA

Gene Name :

Mmp9

Uniprot ID :

P41245

Immunogen :

Expression system for standard: CHO; Immunogen sequence: A20-P730

Cross-reactivity :

There is no detectable cross-reactivity with other relevant proteins.

Assay Range :

156 pg/ml - 10,000 pg/ml

Sensitivity :

<20 pg/ml

Sample Type :

cell culture supernatants, serum and plasma (heparin)

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Gene Full Name :

matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)

Synonyms :

Matrix metalloproteinase-9; MMP-9; 92 kDa gelatinase; 92 kDa type IV collagenase; Gelatinase B; GELB; Mmp9; Clg4b

Protein Name :

Matrix metalloproteinase-9

Molecular Weight :

80535 MW

Protein Function :

Could play a role in bone osteoclastic resorption. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments (By similarity).

Subcellular Localization :

Secreted, extracellular space, extracellular matrix.

Sequence Similarities :

Belongs to the peptidase M10A family.

Background :

The 92-kD type IV collagenase is also known as 92-kD gelatinase, type V collagenase, gelatinase B, or matrix metalloproteinase-9 (MMP9). The 72- and 92-kDa type IV collagenases are members of a group of secreted zinc metalloproteases.1 The matrix metalloproteinases (MMPs) are able to degrade the extracellular matrix and allow angiogenesis and tumor invasion. Gelatinase B, a matrix metalloproteinase that has proteolytic activity against connective tissue proteins, has been suggested to be important in the connective tissue remodeling processes associated with atherogenesis and plaque rupture. MMP-9 is predominantly expressed in neutrophils, macrophages, and mast cells, rather than in oncogene-positive neoplastic cells. The polymorphism of MMP-9 acts as a genetic factor for the development of smoking-induced pulmonary emphysema.

Research Category :

Angiogenesis, Atherosclerosis, Cancer, Cardiovascular, Cell Biology, Cytoskeleton / Ecm, Ecm Enzymes, Extracellular Matrix, Invasion/Microenvironment, Metalloprotease, Proteolysis / Ubiquitin, Proteolytic Enzymes, Signal Transduction, Tumor Biomarkers

more info or order :

Boster product webpage