Human Leptin ELISA Kit EZ-Set™ (DIY Antibody Pairs) | Boster EZ0437 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human Leptin ELISA Kit EZ-Set™ (DIY Antibody Pairs)

catalog :

EZ0437

quantity :

For 5 plates, 96 wells each, reagents only, plates are not included

price :

220 USD

more info or order :

Boster product webpage

citations: 1

Reference
Mozafari N, Robati R, Younespour S. Serum leptin levels in pemphigus: a case control study. Biomed Res Int. 2014;2014:853705 pubmed publisher

image

image 1 :

Human Leptin EZ Set ELISA Kit standard curve

product information

SKU :

EZ0437

Product Name :

Human Leptin ELISA Kit EZ-Set™ (DIY Antibody Pairs)

Size :

For 5 plates, 96 wells each, reagents only, plates are not included

Price :

220 USD

Description :

Human Leptin ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human LEP in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml.

Reactivity :

Human

Application :

ELISA

Gene Name :

LEP

Uniprot ID :

A4D0Y8

Immunogen :

Expression system for standard: E.coli; Immunogen sequence: V22-C167

Cross-reactivity :

There is no detectable cross-reactivity with other relevant proteins.

Assay Range :

62.5 pg/ml - 4,000 pg/ml

Sensitivity :

<10 pg/ml

Sample Type :

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Gene Full Name :

Leptin

Synonyms :

Leptin ;Obesity factor ;LEP ;hCG_33000 , tcag7.84 ;

Protein Name :

Leptin

Molecular Weight :

18641 MW

Protein Function :

May function as part of a signaling pathway that acts to regulate the size of the body fat depot.

Subcellular Localization :

Secreted .

Background :

Leptin (or obese, OB) is a circulating hormone that is expressed abundantly and specifically in the adipose tissue. Human ob protein is a 166-amino acid polypeptide with a putative signal sequence and is 84 and 83% homologous to the mouse and rat ob proteins, respectively. Northern blot analysis reveals that OB RNA is present at a high level in adipose tissue but at much lower levels in placenta and heart. Indeed, Leptin is first described as an adipocyte-derived signalling factor, which, after interaction with its receptors, induces a complex response including control of bodyweight and energy expenditure. Leptin seems in addition to its role in metabolic control to have important roles in reproduction and neuroendocrine signalling. Leptin is a powerful inhibitor of bone formation in vivo. Comparative mapping of mouse and human DNA indicates that the ob gene is located within a region of mouse chromosome 6 that is homologous to a portion of human chromosome 7q. The standard product used in this kit is recombinant human Leptin.

more info or order :

Boster product webpage