ELISA/assay

Human IL-10 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

EZ0416

For 5 plates, 96 wells each, reagents only, plates are not included

220 USD

Human IL-10 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

For 5 plates, 96 wells each, reagents only, plates are not included

220 USD

Human IL-10 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human IL10 in cell culture supernatants, serum and plasma ( heparin, EDTA, citrate). Sensitivity: 0.5 pg/ml.

Human

ELISA

IL10

P22301

Expression system for standard: E.coli; Immunogen sequence: S19-N178

There is no detectable cross-reactivity with other relevant proteins.

7.8 pg/ml - 500 pg/ml (cell culture supernates) 3.4 pg/ml - 250 pg/ml (human serum, plasma)

<0.5 pg/ml

cell culture supernatants, serum and plasma (heparin, EDTA, citrate)

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Interleukin-10

Interleukin-10;IL-10;Cytokine synthesis inhibitory factor;CSIF;IL10;

Interleukin-10

20517 MW

Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells.

Secreted.

Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.

Belongs to the IL-10 family.

Interleukin-10, also called cytokine synthesis inhibitory factor, is implicated in tumorigenesis, and it has been shown that polymorphisms in its gene promoter correlate with differential amounts of production. IL-10 is an important cytokine with anti-inflammatory, anti-immune, and antifibrotic functions. It is also an important regulatory cytokine whose involvement extends into diverse areas of the human immune system. IL-10 is a recently described natural endogenous immunosuppressive cytokine that has been identified in human, murine, and other organisms. IL-10 significantly affects chemokine biology, because human IL-10 inhibits chemokine production and is a specific chemotactic factor for CD8+ T cells. It suppresses the ability of CD4+ T cells, but not CD8+ T cells, to migrate in response to IL-8. Interleukin-10 gene polymorphisms and interleukin-10 production capability may contribute to the development of skin squamous cell carcinomas after renal transplantation.1 The interleukin-10 locus contributes to the heritability of psoriasis susceptibility. With regard to sudden infant death, IL-10 is of special interest. This is an immunoregulatory cytokine that plays an important role in the development of infectious disease The mIL-10 gene is mapped to mouse chromosome 1 and the hIL-10 gene is also mapped to human chromosome 1. The standard product used in this kit is recombinant human IL-10, consisting of 160 amino acids with the molecular mass of 18.6KDa.