ELISA/assay

Human G-CSF ELISA Kit EZ-Set™ (DIY Antibody Pairs)

EZ0360

For 5 plates, 96 wells each, reagents only, plates are not included

255 USD

Human G-CSF ELISA Kit EZ-Set™ (DIY Antibody Pairs)

For 5 plates, 96 wells each, reagents only, plates are not included

255 USD

Human G-CSF ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human CSF3 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 4 pg/ml.

Human

ELISA

CSF3

P09919

Expression system for standard: E.coli; Immunogen sequence: T31-P204

There is no detectable cross-reactivity with other relevant proteins.

31.2 pg/ml - 2,000 pg/ml

<4 pg/ml

cell culture supernatants, serum and plasma (heparin, EDTA, citrate)

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Granulocyte colony-stimulating factor

Granulocyte colony-stimulating factor;G-CSF;Pluripoietin;Filgrastim;Lenograstim;CSF3;C17orf33, GCSF;

Granulocyte colony-stimulating factor

22293 MW

Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.

Secreted.

Belongs to the IL-6 superfamily.

Granulocyte colony-stimulating factor (G-CSF) is a member of the CSF family of hormone-like glycoprotein that regulates hematopoietic cell proliferation and differentiation, and it almost exclusively stimulates the colony formation of granulocytes from committed precursor cells in semi-solid agar culture. G-CSF is also termed colony stimulating factor-3, and a single gene of which codes for a 177 or 180 amino acid mature protein of molecular weight 19,600. Functionally, it specifically stimulates the proliferation and differentiation of the progenitor cells for granulocytes. The effect of G-CSF on myeloid leukemias is unique among colony stimulating factors in driving the leukemic cells from a self-renewing malignant state to a mature differentiated phenotype with the concomitant loss of tumorigenicity. Besides, it also prevents cardiac remodeling after myocardial infarction by activating the Jak-Stat pathway in cardiomyocytes. The recombinant form of hG-CSF is capable of supporting neutrophil proliferation in a CFU-GM assay as well as early erythroid colonies and mixed colony formation. Human gene coding for G-CSF is assigned to the q21-q22 region of chromosome 17. The standard product used in this kit is recombinant human G-CSF, consisting of 175 amino acids with the molecular mass of 18.8KDa.