Human FGF2 ELISA Kit EZ-Set™ (DIY Antibody Pairs) | Boster EZ0342 product information

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product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human FGF2 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

catalog :

EZ0342

quantity :

For 5 plates, 96 wells each

product information

SKU :

EZ0342

Product Name :

Human FGF2 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

Size :

For 5 plates, 96 wells each

Description :

Human FGF2 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human FGF2 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 15 pg/ml. Product Features: Your ideal low cost ELISA solution. EZ Set™ ELISA kit contains the necessary core reagents develop and run the ELISA, including ELISA-optimized matched antibody pairs, recombinant protein standards, and HRP conjugated Avidin-Biotin-Complex (ABC). One kit is sufficient for developing 5 plates (96 well format). EZ Set™ ELISAs are ideal for experienced ELISA users on a budget and great for exploratory work--a fast and easy way to identify the relevant protein targets of interest/relevance to your studies.

Reactivity :

Human

Application :

ELISA

Gene Name :

FGF2

Uniprot ID :

P09038

Immunogen :

Expression system for standard: E.coli; Immunogen sequence: P143-S288

Cross-reactivity :

There is no detectable cross-reactivity with other relevant proteins.

Assay Range :

62.5 pg/ml - 4,000 pg/ml

Sensitivity :

<15 pg/ml

Sample Type :

cell culture supernatants, serum and plasma (heparin, EDTA)

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Synonyms :

Fibroblast growth factor 2; FGF-2; Basic fibroblast growth factor; bFGF; Heparin-binding growth factor 2; HBGF-2; FGF2; FGFB

Protein Name :

Fibroblast growth factor 2

Protein Function :

Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Can induce angiogenesis (PubMed:23469107).

Subcellular Localization :

Secreted. Nucleus. Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57.

Tissue Specificity :

Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non- cancerous liver tissue.

Background :

FGF2 has been implicated in a multitude of physiologic and pathologic processes, including limb development, angiogenesis, wound healing, and tumor growth. Human FGF2 shares 96% and 97% amino acid sequence homology with mouse and rat respectively. FGF2 belongs to the fibroblast growth factor (FGF) family. Fibroblast growth factors (FGFs) exhibit widespread mitogenic and neurotrophic activities. Nine members of the family are currently known, and FGF-1 and FGF-2 are present in relatively high levels in CNS. FGF-2 is expressed by at low levels in many tissues and cell types and reaches high concentrations in brain and pituitary.

Research Category :

Angiogenesis, Cancer, Cardiovascular, Developmental Biology, Growth Factors, Growth Factors/Hormones, Invasion/Microenvironment, Neural Stem Cells, Neurogenesis, Neurology Process, Neuroscience, Organogenesis, Signal Transduction, Stem Cells