Human BDNF EZ-Set ELISA Kit (DIY Antibody Pairs) | Boster EZ0311 product information

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product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human BDNF EZ-Set ELISA Kit (DIY Antibody Pairs)

catalog :

EZ0311

quantity :

For 5 plates, 96 wells each, reagents only, plates are not included

citations: 1

Reference
Koeva Y, Sivkov S, Akabaliev V, Ivanova R, Deneva T, Grozlekova L, et al. Brain-derived neurotrophic factor and its serum levels in schizophrenic patients. Folia Med (Plovdiv). 2014;56:20-3 pubmed

product information

sku :

EZ0311

status :

Enabled

name :

Human BDNF EZ-Set ELISA Kit (DIY Antibody Pairs)

category name :

EZ Set ELISA Kits (Ab Pairs)

gene name :

BDNF

assay range :

31.2pg/ml-2000pg/ml

componentsneeded :

Components Included In The EZ Set Accessory Kit Antibody diluent buffer ABC diluent buffer Sample diluent buffer TMB TMB stop solution 5x 96 well plates, plate sealers . *The EZ Set Accessory Kit provides an easy way to get all the reagents you need for performing an ELISA assay. The components included in the EZ Set Accessory Kit can also be substituted with products other companies if it is of convenience to you. Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

conjugate :

description :

A ELISA kit containing the core components for developing solid phase sandwich ELISA assay to quantitatively detects BMP-2 in Human samples. The kit contains antibody pairs, recombinant protein standards and HRP conjugated ABC.

short description :

description1 :

Product Features: . Your ideal low cost ELISA solution. EZ Set ELISA kit contains the necessary core reagents develop and run the ELISA, including ELISA-optimized matched antibody pairs, recombinant protein standards, and HRP conjugated Avidin-Biotin-Complex (ABC). One kit is sufficient for developing 5 plates (96 well format). EZ Set ELISAs are ideal for experienced ELISA users on a budget and great for exploratory work--a fast and easy way to identify the relevant protein targets of interest/relevance to your studies. . Boster also provides the most comprehensive technical support materials and most user friendly technical support service to guarantee that you can do the experiment smoothly and successfully.

size :

For 5 plates, 96 wells each, reagents only, plates are not included

uniprot id :

P23560

host :

immunogen :

Expression system for standard: sf21; Immunogen sequence: H129-R247

storage :

Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<15pg/ml

kit components :

10ng/tube

sample type :

cell culture supernates, cell lysates, serum and plasma (heparin, EDTA, citrate).

applications :

ELISA

reactivity :

Human

image labels :

Human BMP-2 EZ Set ELISA Kit standard curve Human BMP-2 EZ Set ELISA Kit standard curve

anticoagulant :

heparin, EDTA or citrate

assay procedure step 1 :

2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml

background :

Brain-derived neurotrophic factor(BDNF) is a prosurvival factor induced by cortical neurons that is necessary for survival of striatal neurons in the brain. It is a secreted protein with the molecular weight of 27.8kDa, consisting of 247 amino acids. It is known to promote neuronal survival and differentiation. BDNF shares substantial amino acid sequence identity with nerve growth factor(NGF). BDNF and neurotrophin-3(NT-3) are two recently cloned neurotrophic factors that are homologous to NGF. mRNA products of the BDNF and NT-3 genes are detected in the adult human brain, suggesting that these proteins are involved in the maintenance of the adult nervous system. BDNF and other neurotrophins are critically involved in long-term potentiation(LTP). BDNF-mediated LTP is induced postsynaptically. BDNF has trophic effects on serotonergic(5-HT) neurons in the central nervous system. BDNF has an essential maintenance function in the regulation of anxiety-related behavior and in food intake through central mediators in both the basal and fasted state. It plays a role in treating breathing disorders such as respiratory insufficiency after spinal injury. The mature form of BDNF is identical in all mammals examined, and the gene encoding human BDNF to chromosome 11, band p13.

synonyms :

Brain-derived neurotrophic factor;BDNF;Abrineurin;BDNF;

gene full name :

Brain-derived neurotrophic factor

molecular weight :

27818 MW

protein function :

During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.

subcellular localization :

Secreted.

tissue specificity :

Brain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta.

protein name :

Brain-derived neurotrophic factor

sequence similarities :

Belongs to the NGF-beta family.

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 285 742 1197 306 834 1358 Standard deviation 9.12 25.97 37.12 18.97 58.38 97.78 CV(%) 3.2 3.5 3.1 6.2 7 7.2

sample data :

Concentration(pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.025 0.074 0.101 0.160 0.287 0.563 1.251 2.116

tmb incubation time :

15-25min

principle :

monoclonal antibody from mouse polyclonal antibody from goat

last modified :

5/9/19 5:52