EGFR (Phospho-Ser695) Colorimetric Cell-Based ELISA Kit | Boster EKC2545 product information

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product summary

company name :

Boster

product type :

ELISA/assay

product name :

EGFR (Phospho-Ser695) Colorimetric Cell-Based ELISA Kit

catalog :

EKC2545

quantity :

1 kit, containing 2 plates and all necessary reagents

product information

SKU :

EKC2545

Product Name :

EGFR (Phospho-Ser695) Colorimetric Cell-Based ELISA Kit

Size :

1 kit, containing 2 plates and all necessary reagents

Description :

The EGFR (Phospho-Ser695) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor EGFR (Phospho-Ser695) protein expression profile in cells. The kit can be used for measuring the relative amounts of EGFR (Phospho-Ser695) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on EGFR (Phospho-Ser695).

Reactivity :

Human,Mouse,Rat

Application :

ELISA

Gene Name :

EGFR

Uniprot ID :

P00533

Assay Range :

> 5000 Cells

Sensitivity :

>5000 cells

Storage :

4°C/6 Months

Gene Full Name :

Epidermal growth factor receptor

Synonyms :

Epidermal growth factor receptor;2.7.10.1;Proto-oncogene c-ErbB-1;Receptor tyrosine-protein kinase erbB-1;EGFR;ERBB, ERBB1, HER1;

Molecular Weight :

134277 MW

Protein Function :

Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS- RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin. Plays a role in enhancing learning and memory performance (By similarity).

Subcellular Localization :

Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Golgi apparatus membrane; Single-pass type I membrane protein. Nucleus membrane; Single-pass type I membrane protein. Endosome. Endosome membrane. Nucleus. In response to EGF, translocated from the cell membrane to the nucleus via Golgi and ER. Endocytosed upon activation by ligand. Colocalized with GPER1 in the nucleus of estrogen agonist-induced cancer-associated fibroblasts (CAF).

Tissue Specificity :

Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers.