Human MAG ELISA Kit PicoKine® | Boster EK2061 product information

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product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human MAG ELISA Kit PicoKine®

catalog :

EK2061

quantity :

96 wells/kit, with removable strips.

product information

SKU :

EK2061

Product Name :

Human MAG ELISA Kit PicoKine®

Size :

96 wells/kit, with removable strips.

Description :

Human MAG ELISA Kit PicoKine® (96 Tests). Quantitate Human MAG in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 50pg/ml.

Reactivity :

Human

Application :

ELISA

Gene Name :

MAG

Uniprot ID :

P20916

Immunogen :

Expression system for standard: NS0; Immunogen sequence: G20-P516

Cross-reactivity :

There is no detectable cross-reactivity with other relevant proteins.

Assay Range :

312 pg/ml - 20,000 pg/ml

Sensitivity :

<50 pg/ml

Sample Type :

cell culture supernatants, serum and plasma (heparin, EDTA, citrate)

Capture/Detection Antibodies :

a monoclonal antibody from mouse, a biotinylated polyclonal antibody from goat

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Gene Full Name :

myelin associated glycoprotein

Synonyms :

Myelin-associated glycoprotein; Siglec-4a; MAG; GMA

Protein Function :

Adhesion molecule that mediates interactions between myelinating cells and neurons by binding to neuronal sialic acid-containing gangliosides and to the glycoproteins RTN4R and RTN4RL2. Not required for initial myelination, but seems to play a role in the maintenance of normal axon myelination. Protects motoneurons against apoptosis, also after injury; protection against apoptosis is probably mediated via interaction with neuronal RTN4R and RTN4RL2. Required to prevent degeneration of myelinated axons in adults; this probably depends on binding to gangliosides on the axon cell membrane. Negative regulator of neurite outgrowth; in dorsal root ganglion neurons the inhibition is mediated primarily via binding to neuronal RTN4R or RTN4RL2 and to a lesser degree via binding to neuronal gangliosides. In cerebellar granule cells the inhibition is mediated primarily via binding to neuronal gangliosides. In sensory neurons, inhibition of neurite extension depends only partially on RTN4R, RTN4RL2 and gangliosides. Inhibits axon longitudinal growth. Inhibits axon outgrowth by binding to RTN4R. Preferentially binds to alpha-2,3-linked sialic acid. Binds ganglioside Gt1b.

Subcellular Localization :

Cell membrane. Single-pass type I membrane protein. Membrane raft.

Tissue Specificity :

Both isoform 1 and isoform 2 are detected in myelinated structures in the central and peripheral nervous system, in periaxonal myelin and at Schmidt-Lanterman incisures. Detected in optic nerve, in oligodendroglia and in periaxonal myelin sheaths. Detected in compact myelin. Both isoform 1 and isoform 2 are detected in the central and peripheral nervous system.

Background :

Myelin-associated glycoprotein (MAG, Siglec-4) is a cell membrane glycoprotein that is a member of the SIGLEC family of proteins and is a functional ligand of the NoGo-66 receptor (NgR). This gene is mapped to 19q13.12. The protein encoded by this gene is a type I membrane protein and member of the immunoglobulin superfamily. It is thought to be involved in the process of myelination. It is a lectin that binds to sialylated glycoconjugates and mediates certain myelin-neuron cell-cell interactions. Three alternatively spliced transcripts encoding different isoforms have been described for this gene.