Human Myeloblastin ELISA Kit PicoKine® | Boster EK2034 product information

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product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human Myeloblastin ELISA Kit PicoKine®

catalog :

EK2034

quantity :

96 wells/kit, with removable strips.

product information

SKU :

EK2034

Product Name :

Human Myeloblastin ELISA Kit PicoKine®

Size :

96 wells/kit, with removable strips.

Description :

Human Myeloblastin ELISA Kit PicoKine® (96 Tests). Quantitate Human PRTN3 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml.

Reactivity :

Human

Application :

ELISA

Gene Name :

PRTN3

Uniprot ID :

P24158

Immunogen :

Expression system for standard: E.coli; Immunogen sequence: I28-R248

Cross-reactivity :

There is no detectable cross-reactivity with other relevant proteins.

Assay Range :

62.5 pg/ml - 4,000 pg/ml

Sensitivity :

<10 pg/ml

Sample Type :

cell culture supernatants, serum and plasma (heparin, EDTA)

Capture/Detection Antibodies :

a monoclonal antibody from mouse, a biotinylated polyclonal antibody from goat

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Gene Full Name :

proteinase 3

Synonyms :

Myeloblastin; AGP7; C-ANCA antigen; Leukocyte proteinase 3; PR-3; PR3; Neutrophil proteinase 4; NP-4; P29; Wegener autoantigen; PRTN3; MBN

Protein Name :

Myeloblastin

Protein Function :

Serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro). By cleaving and activating receptor F2RL1/PAR-2, enhances endothelial cell barrier function and thus vascular integrity during neutrophil transendothelial migration. May play a role in neutrophil transendothelial migration, probably when associated with CD177.

Subcellular Localization :

Secreted. Cell membrane. Peripheral membrane protein. Extracellular side. Cytoplasmic granule. Membrane raft. Peripheral membrane protein. Extracellular side.

Tissue Specificity :

Expressed in polymorphonuclear leukocytes (at protein level). Expressed in neutrophils (at protein level). Expressed in differentiating neutrophils.

Background :

Proteinase 3 also known as PRTN3 is an enzyme that in humans is encoded by the PRTN3 gene. This gene is mapped to 19p13.3. PRTN3 is a serine protease enzyme expressed mainly in neutrophil granulocytes. Its exact role in the function of the neutrophil is unknown, but, in human neutrophils, proteinase 3 contributes to the proteolytic generation of antimicrobial peptides. It is also the target of anti-neutrophil cytoplasmic antibodies (ANCAs) of the c-ANCA (cytoplasmic subtype) class, a type of antibody frequently found in the disease granulomatosis with polyangiitis.

Research Category :

Collagen, Cytoskeleton / Ecm, Ecm Enzymes, Ecm Proteins, Extracellular Matrix, Signal Transduction