Human Chitotriosidase/CHIT1 ELISA Kit PicoKine® | Boster EK2032 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human Chitotriosidase/CHIT1 ELISA Kit PicoKine®

catalog :

EK2032

quantity :

96 wells/kit, with removable strips.

product information

SKU :

EK2032

Product Name :

Human Chitotriosidase/CHIT1 ELISA Kit PicoKine®

Size :

96 wells/kit, with removable strips.

Description :

Human Chitotriosidase/CHIT1 ELISA Kit PicoKine® (96 Tests). Quantitate Human CHIT1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml.

Reactivity :

Human

Application :

ELISA

Gene Name :

CHIT1

Uniprot ID :

Q13231

Immunogen :

Expression system for standard: NS0; Immunogen sequence: A22-N466

Cross-reactivity :

There is no detectable cross-reactivity with other relevant proteins.

Assay Range :

312 pg/ml - 20,000 pg/ml

Sensitivity :

<50 pg/ml

Sample Type :

cell culture supernatants, serum and plasma (heparin, EDTA)

Capture/Detection Antibodies :

a monoclonal antibody from mouse, a biotinylated polyclonal antibody from goat

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Gene Full Name :

chitinase 1

Synonyms :

Chitotriosidase-1; Chitinase-1; CHIT1

Protein Function :

Degrades chitin, chitotriose and chitobiose. May participate in the defense against nematodes and other pathogens. Isoform 3 has no enzymatic activity.

Subcellular Localization :

Secreted. Lysosome.

Tissue Specificity :

Detected in spleen. Secreted by cultured macrophages.

Background :

Chitotriosidase encoded by the CHIT1 gene is a member of the chitinase family that is selectively expressed in activated tissue macrophages. This gene is mapped to 1q32.1. Chitotriosidase is secreted by activated human macrophages and is markedly elevated in plasma of Gaucher disease patients. The expression of chitotriosidase occurs only at a late stage of differentiation of monocytes to activated macrophages in culture. Human macrophages can synthesize a functional chitotriosidase, a highly conserved enzyme with a strongly regulated expression. This enzyme may play a role in the degradation of chitin-containing pathogens. Several alternatively spliced transcript variants have been described for this gene.