Human SEMA4A PicoKine ELISA Kit | Boster EK2005 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human SEMA4A PicoKine ELISA Kit

catalog :

EK2005

quantity :

96 wells/kit, with removable strips.

product information

sku :

EK2005

status :

Enabled

name :

Human SEMA4A PicoKine ELISA Kit

category name :

Picokine ELISA Kits

gene name :

SEMA4A

assay range :

62.5pg/ml-4000pg/ml

componentsneeded :

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

conjugate :

description :

Sandwich High Sensitivity ELISA kit for Quantitative Detection of human SEMA4A. 96wells/kit, with removable strips.

short description :

Sandwich High Sensitivity ELISA kit for Quantitative Detection of human SEMA4A. 96wells/kit, with removable strips.

size :

96 wells/kit, with removable strips.

uniprot id :

Q9H3S1

host :

immunogen :

Expression system for standard: NSO, Immunogen sequence: G33-H683

storage :

Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<50pg/ml

kit components :

10ng/tube

sample type :

cell culture supernates, serum and plasma (heparin, EDTA, citrate).

applications :

ELISA

reactivity :

Human

image labels :

Human SEMA4A PicoKine ELISA Kit Standard Curve

anticoagulant :

heparin, EDTA or citrate

assay procedure step 1 :

4000pg/ml, 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml

background :

Semaphorin-4A is a protein that in humans is encoded by the SEMA4A gene. This gene is mapped to 1q22. SEMA4A encodes a member of the semaphorin family of soluble and transmembrane proteins. Semaphorins are involved in numerous functions, including axon guidance, morphogenesis, carcinogenesis, and immunomodulation. The encoded protein is a single-pass type I membrane protein containing an immunoglobulin-like C2-type domain, a PSI domain and a sema domain. It inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons. It is an activator of T-cell-mediated immunity and suppresses vascular endothelial growth factor (VEGF)-mediated endothelial cell migration and proliferation in vitro and angiogenesis in vivo. Mutations in this gene are associated with retinal degenerative diseases including retinitis pigmentosa type 35 (RP35) and cone-rod dystrophy type 10 (CORD10). Multiple alternatively spliced transcript variants encoding different isoforms have been identified.

research category :

Angiogenesis, Axonal Guidance Proteins, Cardiovascular, Growth And Development, Neurology Process, Neuroscience, Sensory System, Visual System

synonyms :

Semaphorin-4A; Semaphorin-B; Sema B; SEMA4A; SEMAB; SEMB; UNQ783; PRO1317

gene full name :

semaphorin 4A

protein function :

Cell surface receptor for PLXNB1, PLXNB2, PLXNB3 and PLXND1 that plays an important role in cell-cell signaling. Plays a role in priming antigen-specific T-cells, promotes differentiation of Th1 T-helper cells, and thereby contributes to adaptive immunity. Promotes phosphorylation of TIMD2. Inhibits angiogenesis. Promotes axon growth cone collapse. Inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons.

subcellular localization :

Cell membrane. Single-pass type I membrane protein.

protein name :

Semaphorin-4A

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 108 392 1205 111 412 1358 Standard deviation 6.37 24.70 73.51 7.33 32.14 108.64 CV(%) 5.9% 6.3% 6.1% 6.6% 7.8% 8.0%

reproducibility :

Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 108 100 105 106 104 2.94 2.8% Sample 2 392 411 420 450 418 20.93 5.0% Sample 3 1205 1248 1148 1303 1226 56.87 4.6% *number of samples for each test n=16.

sample data :

Concentration(pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.033 0.095 0.154 0.269 0.455 0.793 1.315 2.052

tmb incubation time :

15-25min

principle :

a monoclonal antibody from mouse a biotinylated polyclonal antibody from goat

last modified :

3/7/19 6:53