Human GIF ELISA Kit PicoKine® | Boster EK1952 product information

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product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human GIF ELISA Kit PicoKine®

catalog :

EK1952

quantity :

96 wells/kit, with removable strips.

product information

SKU :

EK1952

Product Name :

Human GIF ELISA Kit PicoKine®

Size :

96 wells/kit, with removable strips.

Description :

Human GIF ELISA Kit PicoKine® (96 Tests). Quantitate Human CBLIF in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml.

Reactivity :

Human

Application :

ELISA

Gene Name :

CBLIF

Uniprot ID :

P27352

Immunogen :

Expression system for standard: NS0; Immunogen sequence: S19-Y417

Cross-reactivity :

There is no detectable cross-reactivity with other relevant proteins.

Assay Range :

15.6 pg/ml - 1,000 pg/ml

Sensitivity :

<10 pg/ml

Sample Type :

cell culture supernatants, serum and plasma (heparin, EDTA, citrate)

Capture/Detection Antibodies :

a monoclonal antibody from mouse, a biotinylated polyclonal antibody from goat

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Gene Full Name :

cobalamin binding intrinsic factor

Synonyms :

Cobalamin binding intrinsic factor; Gastric intrinsic factor; Intrinsic factor; IF; INF; CBLIF; GIF

Protein Function :

Promotes absorption of the essential vitamin cobalamin (Cbl) in the ileum. After interaction with CUBN, the CBLIF-cobalamin complex is internalized via receptor-mediated endocytosis.

Subcellular Localization :

Secreted.

Tissue Specificity :

Gastric mucosa

Background :

Intrinsic factor (IF), also known as gastric intrinsic factor (GIF), is a glycoprotein produced by the parietal cells of the stomach. It is necessary for the absorption of vitamin B12 later on in the ileum of the small intestine. In humans, the gastric intrinsic factor protein is encoded by the GIF gene. It is mapped to 11q12.1. Haptocorrin (also known as HC, R protein, and transcobalamin I, TCN1) is another glycoprotein secreted by the salivary glands which binds to vitamin B12. Vitamin B12 is acid sensitive and in binding to transcobalamin I it can safely pass through the acidic stomach to the duodenum. In the less acidic environment of the small intestine, pancreatic enzymes digest the glycoprotein carrier and vitamin B12 can then bind to intrinsic factor