ELISA/assay

Human CD55 ELISA Kit PicoKine®

EK1948

96 wells/kit, with removable strips.

Human CD55 ELISA Kit PicoKine®

96 wells/kit, with removable strips.

Human CD55 ELISA Kit PicoKine® (96 Tests). Quantitate Human CD55 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml.

Human

ELISA

CD55

P08174

Expression system for standard: NS0; Immunogen sequence: D35-S353

There is no detectable cross-reactivity with other relevant proteins.

312 pg/ml - 20,000 pg/ml

<50 pg/ml

cell culture supernatants, serum and plasma (heparin, EDTA)

A monoclonal antibody from mouse, a biotinylated detection polyclonal antibody from goat

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

CD55 molecule (Cromer blood group)

Complement decay-accelerating factor; CD55; CR; DAF

This protein recognizes C4b and C3b fragments that condense with cell-surface hydroxyl or amino groups when nascent C4b and C3b are locally generated during C4 and c3 activation. Interaction of daf with cell-associated C4b and C3b polypeptides interferes with their ability to catalyze the conversion of C2 and factor B to enzymatically active C2a and Bb and thereby prevents the formation of C4b2a and C3bBb, the amplification convertases of the complement cascade. Inhibits complement activation by destabilizing and preventing the formation of C3 and C5 convertases, which prevents complement damage.

Cell membrane, Single-pass type I membrane protein, GPI-anchor,Secreted

Expressed on the plasma membranes of all cell types that are in intimate contact with plasma complement proteins. It is also found on the surfaces of epithelial cells lining extracellular compartments, and variants of the molecule are present in body fluids and in extracellular matrix.

Complement decay-accelerating factor, also known as CD55 or DAF, is a protein that, in humans, is encoded by the CD55 gene. It is mapped to 1q32.2. This gene encodes a glycoprotein involved in the regulation of the complement cascade. Binding of the encoded protein to complement proteins accelerates their decay, thereby disrupting the cascade and preventing damage to host cells. Antigens present on this protein constitute the Cromer blood group system (CROM). Alternative splicing results in multiple transcript variants. The predominant transcript variant encodes a membrane-bound protein, but alternatively spliced transcripts may produce soluble proteins.

Cell Type Markers, Immunology