ELISA/assay

Human B4GALT1 ELISA Kit PicoKine®

EK1880

96 wells/kit, with removable strips.

Human B4GALT1 ELISA Kit PicoKine®

96 wells/kit, with removable strips.

Human B4GALT1 ELISA Kit PicoKine® (96 Tests). Quantitate Human B4GALT1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 0.1ng/ml.

Human

ELISA

B4GALT1

P15291

Expression system for standard: NS0; Immunogen sequence: G44-S398

There is no detectable cross-reactivity with other relevant proteins.

0.78 ng/ml - 50 ng/ml

<50 pg/ml

cell culture supernatants, serum and plasma (heparin, EDTA)

A monoclonal antibody from mouse, a biotinylated detection polyclonal antibody from goat

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

beta-1,4-galactosyltransferase 1

Beta-1,4-galactosyltransferase 1; Beta-1,4-GalTase 1; Beta4Gal-T1; beta-GlcNAc beta-1,4-galactosyltransferase 1; beta-N-acetylglucosamine beta-1,4-galactosyltransferase 1;

The Golgi complex form catalyzes the production of lactose in the lactating mammary gland and could also be responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.

Cell membrane, Single-pass type II membrane protein, Golgi stack membrane, Cell surface, filopodium, Golgi stack membrane, Secreted

Ubiquitously expressed, but at very low levels in fetal and adult brain.

Beta-1,4-galactosyltransferase 1 is an enzyme that in humans is encoded by the B4GALT1 gene. It is mapped to 9p21.1. This gene is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose; all transfer galactose in a beta1,4 linkage to similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. This gene is unique among the beta4GalT genes because it encodes an enzyme that participates both in glycoconjugate and lactose biosynthesis. For the first activity, the enzyme adds galactose to N-acetylglucosamine residues that are either monosaccharides or the nonreducing ends of glycoprotein carbohydrate chains. The second activity is restricted to lactating mammary tissues where the enzyme forms a heterodimer with alpha-lactalbumin to catalyze UDP-galactose + D-glucose UDP + lactose. The two enzymatic forms result from alternate transcription initiation sites and post-translational processing. Two transcripts, which differ only at the 5' end, with approximate lengths of 4.1 kb and 3.9 kb encode the same protein. The longer transcript encodes the type II membrane-bound, trans-Golgi resident protein involved in glycoconjugate biosynthesis. The shorter transcript encodes a protein which is cleaved to form the soluble lactose synthase.

Developmental Biology, Lipid Metabolism, Metabolism, Reproduction, Signal Transduction