Human Haptoglobin ELISA Kit PicoKine® | Boster EK1702 product information

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product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human Haptoglobin ELISA Kit PicoKine®

catalog :

EK1702

quantity :

96 wells/kit, with removable strips.

product information

SKU :

EK1702

Product Name :

Human Haptoglobin ELISA Kit PicoKine®

Size :

96 wells/kit, with removable strips.

Description :

Human Haptoglobin ELISA Kit PicoKine® (96 Tests). Quantitate Human HP in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 20pg/ml .

Reactivity :

Human

Application :

ELISA

Gene Name :

Uniprot ID :

P00738

Immunogen :

Expression system for standard: NS0; Immunogen sequence: V19-N406

Cross-reactivity :

There is no detectable cross-reactivity with other relevant proteins.

Assay Range :

312 pg/ml - 20,000 pg/ml

Sensitivity :

<20 pg/ml

Sample Type :

cell culture supernatants, serum and plasma (heparin, EDTA)

Capture/Detection Antibodies :

a monoclonal antibody from mouse, a biotinylated polyclonal antibody from goat

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Gene Full Name :

haptoglobin

Synonyms :

Haptoglobin; Zonulin; Haptoglobin alpha chain; Haptoglobin beta chain; HP

Protein Function :

As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an Antimicrobial; Antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidely cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway.

Subcellular Localization :

Secreted.

Tissue Specificity :

Expressed by the liver and secreted in plasma.

Background :

Haptoglobin (HP), is a protein that in humans is encoded by the HP gene. Haptoglobin, a plasma glycoprotein that binds free hemoglobin, has a tetrameric structure of 2 alpha and 2 beta polypeptides that are covalently associated by disulfide bonds. Haptoglobin is homologous to serine proteases of the chymotrypsinogen family. In the chimpanzee, there are 3 genes in the haptoglobin family (haptoglobin, HP; haptoglobin-related, HPR; and haptoglobin-primate, HPP), whereas only 2 genes exist in humans (HP and HPR). There are similarities between the primary structures of the alpha chain and of light chains of gamma globulins. The alpha haptoglobin locus is on the long arm of chromosome 16, where its exact cytogenetic location is 16q22.2. A major function of haptoglobin is to bind hemoglobin (Hb) to form a stable Hp-Hb complex and thereby prevent Hb-induced oxidative tissue damage. Haptoglobin is an unusual secretory protein in that it is proteolytically processed in the endoplasmic reticulum and not in the Golgi. The human haptoglobin HP*2 allele contains a 1.7-kb intragenic duplication that arose after a unique nonhomologous recombination between the prototype HP*1 alleles.

Research Category :

Tags & Cell Markers