ELISA/assay

Human MCAM/CD146 ELISA Kit PicoKine®

EK1675

96 wells/kit, with removable strips.

Human MCAM/CD146 ELISA Kit PicoKine®

96 wells/kit, with removable strips.

Human MCAM/CD146 ELISA Kit PicoKine® (96 Tests). Quantitate Human MCAM in cell culture supernatants and serum. Sensitivity: 10pg/ml.

Human

ELISA

MCAM

P43121

Expression system for standard: NS0; Immunogen sequence: V24-G559

There is no detectable cross-reactivity with other relevant proteins.

62.5 pg/ml - 4,000 pg/ml

<10 pg/ml

cell culture supernatants and serum

a monoclonal antibody from mouse, a biotinylated polyclonal antibody from goat

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

melanoma cell adhesion molecule

Cell surface glycoprotein MUC18; Cell surface glycoprotein P1H12; Melanoma cell adhesion molecule; Melanoma-associated antigen A32; Melanoma-associated antigen MUC18; S-endo 1 endothelial-associated antigen; CD146; MCAM; MUC18

Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration.

Membrane; Single-pass type I membrane protein.

Detected in endothelial cells in vascular tissue throughout the body. May appear at the surface of neural crest cells during their embryonic migration. Appears to be limited to vascular smooth muscle in normal adult tissues. Associated with tumor progression and the development of metastasis in human malignant melanoma. Expressed most strongly on metastatic lesions and advanced primary tumors and is only rarely detected in benign melanocytic nevi and thin primary melanomas with a low probability of metastasis.

CD146 (cluster of differentiation 146) also known as the melanoma cell adhesion molecule (MCAM) or cell surface glycoprotein MUC18, is a 113kDa cell adhesion molecule currently used as a marker for endothelial cell lineage. In humans, the CD146 protein is encoded by the MCAM gene. By radiation hybrid analysis, it mapped the CD146 gene to chromosome 11q23.3.It is a member of the immunoglobulin superfamily, is homologous to several cell adhesion molecules and is associated with tumor progression and the development of metastasis in human malignant melanoma. CD146 has been demonstrated to appear on a small subset of T and B lymphocytes in the peripheral blood of healthy individuals. The CD146+ T cells display an immunophenotype consistent with effector memory cells and have a distinct gene profile from the CD146- T cells.CD146 has been seen as a marker for mesenchymal stem cells isolated from multiple adult and fetal organs, and its expression may be linked to multipotency; mesenchymal stem cells with greater differentiation potential express higher levels of CD146 on the cell surface.

Cancer, Cell Adhesion, Cell Adhesion Molecules, Cell Type Markers, Cytoskeleton / Ecm, Immunology, Invasion/Microenvironment, Signal Transduction, Stem Cells