Human Autotaxin ELISA Kit PicoKine® | Boster EK1655 product information

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product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human Autotaxin ELISA Kit PicoKine®

catalog :

EK1655

quantity :

96 wells/kit, with removable strips.

product information

SKU :

EK1655

Product Name :

Human Autotaxin ELISA Kit PicoKine®

Size :

96 wells/kit, with removable strips.

Description :

Human Autotaxin ELISA Kit PicoKine® (96 Tests). Quantitate Human ENPP2 in cell culture supernatants, serum, plasma (heparin) , urine and human milk. Sensitivity: 10pg/ml.

Reactivity :

Human

Application :

ELISA

Gene Name :

ENPP2

Uniprot ID :

Q13822

Immunogen :

Expression system for standard: NS0; Immunogen sequence: A36-I863

Cross-reactivity :

There is no detectable cross-reactivity with other relevant proteins.

Assay Range :

625 pg/ml - 40,000 pg/ml

Sensitivity :

<10 pg/ml

Sample Type :

cell culture supernatants, serum, plasma (heparin) , urine and human milk

Capture/Detection Antibodies :

a monoclonal antibody from mouse, a biotinylated polyclonal antibody from goat

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Gene Full Name :

ectonucleotide pyrophosphatase/phosphodiesterase 2

Synonyms :

Ectonucleotide pyrophosphatase/phosphodiesterase family member 2; E-NPP 2; Autotaxin; Extracellular lysophospholipase D; LysoPLD; ENPP2; ATX; PDNP2

Protein Function :

Hydrolyzes lysophospholipids to produce lysophosphatidic acid (LPA) in extracellular fluids. Major substrate is lysophosphatidylcholine. Also can act on sphingosylphosphphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility. Can hydrolyze, in vitro, bis-pNPP, to some extent pNP-TMP, and barely ATP. Involved in several motility- related processes such as angiogenesis and neurite outgrowth. Acts as an angiogenic factor by stimulating migration of smooth muscle cells and microtubule formation. Stimulates migration of melanoma cells, probably via a pertussis toxin-sensitive G protein. May have a role in induction of parturition. Possible involvement in cell proliferation and adipose tissue development. Tumor cell motility-stimulating factor.

Subcellular Localization :

Secreted.

Tissue Specificity :

Predominantly expressed in brain, placenta, ovary, and small intestine. Expressed in a number of carcinomas such as hepatocellular and prostate carcinoma, neuroblastoma and non-small-cell lung cancer. Expressed in body fluids such as plasma, cerebral spinal fluid (CSF), saliva, follicular and amniotic fluids. Not detected in leukocytes. Isoform 1 is more highly expressed in peripheral tissues than in the central nervous system (CNS). Adipocytes only express isoform 1. Isoform 3 is more highly expressed in the brain than in peripheral tissues.

Background :

Autotaxin, also known as ectonucleotide pyrophosphatase/phosphodiesterase family member 2 (E-NPP 2), is an enzyme that in humans is encoded by the ENPP2 gene. The protein encoded by this gene functions as both a phosphodiesterase, which cleaves phosphodiester bonds at the 5' end of oligonucleotides, and a phospholipase, which catalyzes production of lysophosphatidic acid (LPA) in extracellular fluids. LPA evokes growth factor-like responses including stimulation of cell proliferation and chemotaxis. This gene product stimulates the motility of tumor cells and has angiogenic properties, and its expression is upregulated in several kinds of carcinomas. The gene product is secreted and further processed to make the biologically active form. Several alternatively spliced transcript variants encoding different isoforms have been identified.

Research Category :

Metabolism, Neurodegenerative Disease, Neurology Process, Neuroscience, Pathways And Processes, Signal Transduction, Tumor Biomarkers