Human Serpin A1/alpha 1-Antitrypsin ELISA Kit PicoKine®

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product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human Serpin A1/alpha 1-Antitrypsin ELISA Kit PicoKine®

catalog :

EK1634

quantity :

96 wells/kit, with removable strips.

product information

SKU :

EK1634

Product Name :

Human Serpin A1/alpha 1-Antitrypsin ELISA Kit PicoKine®

Size :

96 wells/kit, with removable strips.

Description :

Human Serpin A1/alpha 1-Antitrypsin ELISA Kit PicoKine® (96 Tests). Quantitate Human SERPINA1 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 10pg/ml .

Reactivity :

Human

Application :

ELISA

Gene Name :

SERPINA1

Uniprot ID :

P01009

Immunogen :

Expression system for standard: NS0; Immunogen sequence: E25-K418

Cross-reactivity :

There is no detectable cross-reactivity with other relevant proteins.

Assay Range :

1.56 ng/ml - 100 ng/ml

Sensitivity :

<10 pg/ml

Sample Type :

cell culture supernatants, serum, plasma (heparin, EDTA) and urine

Capture/Detection Antibodies :

a monoclonal antibody from mouse, a biotinylated polyclonal antibody from goat

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Gene Full Name :

serpin family A member 1

Synonyms :

Alpha-1-antitrypsin; Alpha-1 protease inhibitor; Alpha-1-antiproteinase; Serpin A1; Short peptide from AAT; SPAAT; SERPINA1; AAT; PI; PRO0684; PRO2209

Protein Function :

Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.

Subcellular Localization :

Secreted. Endoplasmic reticulum. The S and Z allele are not secreted effectively and accumulate intracellularly in the endoplasmic reticulum.

Tissue Specificity :

Ubiquitous. Expressed in leukocytes and plasma.

Background :

Alpha-1-antitrypsin or α1-antitrypsin (A1AT, A1A, or AAT) is a protein belonging to the serpin superfamily. It is encoded in humans by the SERPINA1 gene. The gene is located on the long arm of the fourteenth chromosome (14q32.1).The protein encoded by this gene is secreted and is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Defects in this gene can cause emphysema or liver disease. Several transcript variants encoding the same protein have been found for this gene.Like all serine protease inhibitors, A1AT has a characteristic secondary structure of beta sheets and alpha helices. Mutations in these areas can lead to non-functional proteins that can polymerise and accumulate in the liver (infantile hepatic cirrhosis).

Research Category :

Intracellular, Kinases, Metabolism, Metabolism Processes, Pathways And Processes, Protease Inhibitors, Proteolysis / Ubiquitin, Response To Hypoxia, Serum Proteins