Human Legumain(total) ELISA Kit PicoKine® | Boster EK1566 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human Legumain(total) ELISA Kit PicoKine®

catalog :

EK1566

quantity :

96 wells/kit, with removable strips.

product information

SKU :

EK1566

Product Name :

Human Legumain(total) ELISA Kit PicoKine®

Size :

96 wells/kit, with removable strips.

Description :

Human Legumain (total) ELISA Kit PicoKine® (96 Tests). Quantitate Human LGMN in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Reactivity :

Human

Application :

ELISA

Gene Name :

LGMN

Uniprot ID :

Q99538

Immunogen :

Expression system for standard: NS0; Immunogen sequence: V18-Y433

Cross-reactivity :

There is no detectable cross-reactivity with other relevant proteins.

Assay Range :

31.2 pg/ml - 2,000 pg/ml

Sensitivity :

<10 pg/ml

Sample Type :

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Gene Full Name :

Legumain

Synonyms :

Legumain;3.4.22.34;Asparaginyl endopeptidase;Protease, cysteine 1;LGMN;PRSC1;

Protein Name :

Legumain

Molecular Weight :

49411 MW

Protein Function :

Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. Required for normal lysosomal protein degradation in renal proximal tubules. Required for normal degradation of internalized EGFR. Plays a role in the regulation of cell proliferation via its role in EGFR degradation (By similarity). May be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system.

Subcellular Localization :

lysosome

Tissue Specificity :

Ubiquitous. Particularly abundant in kidney, heart and placenta.

Background :

Legumain (asparaginyl endopeptidase, citvac, proteinase B, hemoglobinase, PRSC1 gene product or LGMN (Homo sapiens), vicilin peptidohydrolase, bean endopeptidase) is an enzyme that in humans is encoded by the LGMN gene (previous symbolPRSC1). Using fluorescence in situ hybridization, the LGMN gene was mapped to chromosome 14q32.1. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic. Protein expression occurs after monocytes differentiate into dendritic cells. A fully mature, active enzyme is produced following lipopolysaccharide expression in mature dendritic cells. Overexpression of this gene may be associated with the majority of solid tumor types.

Research Category :

Cancer, Cancer Metabolism, Cell Biology, Cell Type Markers, Cytoskeleton / Ecm, Endocrine Metabolism, Extracellular Matrix, Hormone Biosynthesis, Immunology, Metabolic Signaling Pathway, Metabolism, Non-Cd, Pathways And Processes, Proteolysis / Ubiquitin, Proteolytic Enzymes, Signal Transduction, Structures