Human Pan Trypsin(PRSS1/2/3) ELISA Kit PicoKine® | Boster EK1531 product information

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product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human Pan Trypsin(PRSS1/2/3) ELISA Kit PicoKine®

catalog :

EK1531

quantity :

96 wells/kit, with removable strips.

product information

SKU :

EK1531

Product Name :

Human Pan Trypsin(PRSS1/2/3) ELISA Kit PicoKine®

Size :

96 wells/kit, with removable strips.

Description :

Human Pan Trypsin (PRSS1/2/3) ELISA Kit PicoKine® (96 Tests). Quantitate Human PRSS1/PRSS2/PRSS3 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10pg/ml.

Reactivity :

Human

Application :

ELISA

Gene Name :

PRSS1/PRSS2/PRSS3

Uniprot ID :

P07477/P07478/P35030

Immunogen :

Expression system for standard: NS0; Immunogen sequence: A16-S247

Cross-reactivity :

There is no detectable cross-reactivity with other relevant proteins.

Assay Range :

31.2 pg/ml - 2,000 pg/ml

Sensitivity :

<10 pg/ml

Sample Type :

cell culture supernatants, serum and plasma (heparin)

Capture/Detection Antibodies :

monoclonal antibody from mouse, polyclonal antibody from goat

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Gene Full Name :

protease, serine 1/protease, serine 2/protease, serine 3

Synonyms :

Trypsin-3;3.4.21.4;Brain trypsinogen;Mesotrypsinogen;Serine protease 3;Serine protease 4;Trypsin III;Trypsin IV;PRSS3;PRSS4, TRY3, TRY4;

Protein Name :

Trypsin-1/Trypsin-2/Trypsin-3

Molecular Weight :

32529 MW

Protein Function :

Digestive protease specialized for the degradation of trypsin inhibitors. In the ileum, may be involved in defensin processing, including DEFA5.

Subcellular Localization :

Secreted.

Tissue Specificity :

Expressed in pancreas and brain. Also expressed in Paneth cells, at the base of small intestinal crypts.

Background :

Trypsin is a serine protease from the PA clan superfamily, found in the digestive system of many vertebrates, where it hydrolyses proteins. In the duodenum, trypsin catalyzes the hydrolysis of peptide bonds, breaking down proteins into smaller peptides. The peptide products are then further hydrolyzed into amino acids via other proteases, rendering them available for absorption into the blood stream. Tryptic digestion is a necessary step in protein absorption as proteins are generally too large to be absorbed through the lining of the small intestine. Additionally, Trypsin is produced as the inactive zymogen trypsinogen in the pancreas. When the pancreas is stimulated by cholecystokinin, it is then secreted into the first part of the small intestine (the duodenum) via the pancreatic duct. Once in the small intestine, the enzyme enteropeptidase activates trypsinogen into trypsin by proteolytic cleavage. Auto catalysis can happen with trypsin using trypsinogen as the substrate. This activation mechanism is common for most serine proteases, and serves to prevent autodegradation of the pancreas.

Research Category :

Cancer, Cell Biology, Proteolysis / Ubiquitin, Proteolytic Enzymes, Tumor Biomarkers