Human R-Spondin-1 ELISA Kit PicoKine® | Boster EK1516 product information

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product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human R-Spondin-1 ELISA Kit PicoKine®

catalog :

EK1516

quantity :

96 wells/kit, with removable strips.

product information

SKU :

EK1516

Product Name :

Human R-Spondin-1 ELISA Kit PicoKine®

Size :

96 wells/kit, with removable strips.

Description :

Human R-Spondin-1 ELISA Kit PicoKine® (96 Tests). Quantitate Human RSPO1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Reactivity :

Human

Application :

ELISA

Gene Name :

RSPO1

Uniprot ID :

Q2MKA7

Immunogen :

Expression system for standard: NS0; Immunogen sequence: S21-A263

Cross-reactivity :

There is no detectable cross-reactivity with other relevant proteins.

Assay Range :

31.2 pg/ml - 2,000 pg/ml

Sensitivity :

<10 pg/ml

Sample Type :

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Capture/Detection Antibodies :

polyclonal antibody from goat, polyclonal antibody from goat

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Gene Full Name :

R-spondin-1

Synonyms :

R-spondin-1;Roof plate-specific spondin-1;hRspo1;RSPO1;

Protein Name :

R-spondin-1

Molecular Weight :

28959 MW

Protein Function :

Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Has a essential roles in ovary determination.

Subcellular Localization :

Secreted . Nucleus . Seems to mainly localize to nucleoli.

Tissue Specificity :

Abundantly expressed in adrenal glands, ovary, testis, thyroid and trachea but not in bone marrow, spinal cord, stomach, leukocytes colon, small intestine, prostate, thymus and spleen.

Background :

R-spondin-1 is a secreted protein that in humans is encoded by the Rspo1 gene. It is mapped to chromosome 1p34. This gene encodes a secreted activator protein with two cysteine-rich, furin-like domains and one thrombospondin type 1 domain. The encoded protein is a ligand for leucine-rich repeat-containing G-protein coupled receptors (LGR proteins) and positively regulates the Wnt signaling pathway. In mice, the protein induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy- induced adverse effects. Alternative splicing results in multiple transcript variants.

Research Category :

Reproduction, Secreted, Signaling Pathways, Stem Cells