Human R-Spondin-3 ELISA Kit PicoKine® | Boster EK1512 product information

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product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human R-Spondin-3 ELISA Kit PicoKine®

catalog :

EK1512

quantity :

96 wells/kit, with removable strips.

product information

SKU :

EK1512

Product Name :

Human R-Spondin-3 ELISA Kit PicoKine®

Size :

96 wells/kit, with removable strips.

Description :

Human R-Spondin-3 ELISA Kit PicoKine® (96 Tests). Quantitate Human RSPO3 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Reactivity :

Human

Application :

ELISA

Gene Name :

RSPO3

Uniprot ID :

Q9BXY4

Immunogen :

Expression system for standard: CHO; Immunogen sequence: Q22-H272

Cross-reactivity :

There is no detectable cross-reactivity with other relevant proteins.

Assay Range :

15.6 pg/ml - 1,000 pg/ml

Sensitivity :

<10 pg/ml

Sample Type :

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Capture/Detection Antibodies :

polyclonal antibody from sheep, polyclonal antibody from sheep

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Gene Full Name :

R-spondin-3

Synonyms :

R-spondin-3;Protein with TSP type-1 repeat;hPWTSR;Roof plate-specific spondin-3;hRspo3;Thrombospondin type-1 domain-containing protein 2;RSPO3;PWTSR, THSD2;

Protein Name :

R-spondin-3

Molecular Weight :

30929 MW

Protein Function :

Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors, which acts as a key regulator of angiogenesis. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta- catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway (PubMed:21727895, PubMed:21909076, PubMed:22615920). Acts as a key regulator of angiogenesis by controlling vascular stability and pruning: acts by activating the non-canonical Wnt signaling pathway in endothelial cells (By similarity).

Subcellular Localization :

Secreted .

Tissue Specificity :

Ubiquitously expressed. Expressed at higher level in placenta, small intestine, fetal thymus and lymph node (PubMed:12463421). Highly expressed in endothelial cells (PubMed:26766444).

Background :

R-spondin-3 is a protein that in humans is encoded by the RSPO3 gene. This gene belongs to the R-spondin family. The encoded protein plays a role in the regulation of Wnt (wingless-type MMTV integration site family)/beta-catenin and Wnt/planar cell polarity (PCP) signaling pathways, which are involved in development, cell growth and disease pathogenesis. Genome-wide association studies suggest a correlation of this gene with bone mineral density and risk of fracture. This gene may be involved in tumor development.

Research Category :

Developmental Biology, Reproduction, Secreted, Signaling Pathways, Stem Cells