Human SPHK1/Sphingosine Kinase 1 ELISA Kit PicoKine® | Boster EK1501 product information

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product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human SPHK1/Sphingosine Kinase 1 ELISA Kit PicoKine®

catalog :

EK1501

quantity :

96 wells/kit, with removable strips.

product information

SKU :

EK1501

Product Name :

Human SPHK1/Sphingosine Kinase 1 ELISA Kit PicoKine®

Size :

96 wells/kit, with removable strips.

Description :

Human SPHK1/Sphingosine Kinase 1 ELISA Kit PicoKine® (96 Tests). Quantitate Human SPHK1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Reactivity :

Human

Application :

ELISA

Gene Name :

SPHK1

Uniprot ID :

Q9NYA1

Immunogen :

Expression system for standard: NS0; Immunogen sequence: M1-L384

Cross-reactivity :

There is no detectable cross-reactivity with other relevant proteins.

Assay Range :

312 pg/ml - 20,000 pg/ml

Sensitivity :

<10 pg/ml

Sample Type :

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Gene Full Name :

Sphingosine kinase 1

Synonyms :

Sphingosine kinase 1;SK 1;SPK 1;2.7.1.91;SPHK1;SPHK, SPK;

Protein Name :

Sphingosine kinase 1

Molecular Weight :

42518 MW

Protein Function :

Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol.

Subcellular Localization :

Cytoplasm. Nucleus. Cell membrane. Translocated from the cytoplasm to the plasma membrane in a CIB1-dependent manner.

Tissue Specificity :

Widely expressed with highest levels in adult liver, kidney, heart and skeletal muscle.

Background :

Sphingosine kinase 1 is an enzyme that in humans is encoded by the SPHK1 gene. The protein encoded by this gene catalyzes the phosphorylation of sphingosine to form sphingosine-1- phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Intracellularly, S1P regulates proliferation and survival, and extracellularly, it is a ligand for cell surface G protein-coupled receptors. This protein, and its product S1P, play a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Research Category :

Cancer, Cancer Metabolism, G Protein Signaling, Lipid And Lipoprotein Metabolism, Lipid Metabolism, Lipid Signaling, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Metabolism Of Lipids And Lipoproteins, Pathways And Processes, Signal Transduction, Signaling Pathway