Human Klotho beta/KLB ELISA Kit PicoKine® | Boster EK1492 product information

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product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human Klotho beta/KLB ELISA Kit PicoKine®

catalog :

EK1492

quantity :

96 wells/kit, with removable strips.

product information

SKU :

EK1492

Product Name :

Human Klotho beta/KLB ELISA Kit PicoKine®

Size :

96 wells/kit, with removable strips.

Description :

Human Klotho beta ELISA Kit PicoKine® (96 Tests). Quantitate Human KLB in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml.

Reactivity :

Human

Application :

ELISA

Gene Name :

KLB

Uniprot ID :

Q86Z14

Immunogen :

Expression system for standard: NS0; Immunogen sequence: F53-L997

Cross-reactivity :

There is no detectable cross-reactivity with other relevant proteins.

Assay Range :

156 pg/ml - 10,000 pg/ml

Sensitivity :

<50 pg/ml

Sample Type :

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Capture/Detection Antibodies :

monoclonal antibody from mouse, polyclonal antibody from goat

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Gene Full Name :

Beta-klotho

Synonyms :

Beta-klotho;BKL;BetaKlotho;Klotho beta-like protein;KLB;

Protein Name :

Beta-klotho

Molecular Weight :

119808 MW

Protein Function :

Contributes to the transcriptional repression of cholesterol 7-alpha-hydroxylase (CYP7A1), the rate-limiting enzyme in bile acid synthesis. Probably inactive as a glycosidase. Increases the ability of FGFR1 and FGFR4 to bind FGF21 (By similarity).

Subcellular Localization :

Cell membrane ; Single-pass type III membrane protein .

Background :

Klotho beta, a divergent structural member of the glycosidase I superfamily, is expressed primarily in the liver and pancreas, with lower expression in adipose tissue. It is mapped to chromosome 4. The Klotho beta mediated interaction of human FGF19 (mouse FGF15) with FGF Receptor 4 in the liver negatively regulates bile acid synthesis by controlling the secretion of two key bile acid synthase genes, cholesterol 7-alpha hydroxylase (Cyp7a1) and sterol 12-alpha hydroxylase (Cyp8b1). Klotho beta is also an essential cofactor for FGF21 activity and the Klotho gene family may have evolved to confer tissue-specific bioactivity on FGF19 subfamily members.

Research Category :

Cancer, Cardiovascular, Cholesterol Metabolism, Energy Metabolism, Energy Transfer Pathways, Epigenetics and Nuclear Signaling, Growth Factors/Hormones, Lipid And Lipoprotein Metabolism, Lipid Metabolism, Lipids / Lipoproteins, Metabolic Signaling Pathways, Metabolism, Pathways And Processes, Signal Transduction, Transcription