Human PD-L1/B7-H1 ELISA Kit PicoKine® | Boster EK1449 product information

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product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human PD-L1/B7-H1 ELISA Kit PicoKine®

catalog :

EK1449

quantity :

96 wells/kit, with removable strips.

product information

SKU :

EK1449

Product Name :

Human PD-L1/B7-H1 ELISA Kit PicoKine®

Size :

96 wells/kit, with removable strips.

Description :

Human PD-L1/B7-H1 ELISA Kit PicoKine® (96 Tests). Quantitate Human CD274 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 12pg/ml.

Reactivity :

Human

Application :

ELISA

Gene Name :

CD274

Uniprot ID :

Q9NZQ7

Immunogen :

Expression system for standard: NS0; Immunogen sequence: F19-R238

Cross-reactivity :

There is no detectable cross-reactivity with other relevant proteins.

Assay Range :

62.5 pg/ml - 4,000 pg/ml

Sensitivity :

<12 pg/ml

Sample Type :

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Capture/Detection Antibodies :

polyclonal antibody from goat, polyclonal antibody from goat

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Gene Full Name :

Programmed cell death 1 ligand 1

Synonyms :

Programmed cell death 1 ligand 1;PD-L1;PDCD1 ligand 1;Programmed death ligand 1;B7 homolog 1;B7-H1;CD274;CD274;B7H1, PDCD1L1, PDCD1LG1, PDL1;

Protein Name :

Programmed cell death 1 ligand 1

Molecular Weight :

33275 MW

Protein Function :

Involved in the costimulatory signal, essential for T- cell proliferation and production of IL10 and IFNG, in an IL2- dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production.

Subcellular Localization :

Isoform 1: Cell membrane; Single-pass type I membrane protein.

Tissue Specificity :

Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes.

Background :

Programmed death-ligand 1 (PD-L1), also known as cluster of differentiation 274 (CD274) or B7 homolog 1 (B7-H1), is a protein that in humans is encoded by the CD274 gene. PD-L1 is a 40kDa type 1 transmembrane protein that has been speculated to play a major role in suppressing the immune system during particular events such as pregnancy, tissue allografts, autoimmune disease and other disease states such as hepatitis. Normally the immune system reacts to foreign antigens where there is some accumulation in the lymph nodes or spleen which triggers a proliferation of antigen-specific CD8+ T cell. The formation of PD-1 receptor / PD-L1 or B7.1 receptor /PD-L1 ligand complex transmits an inhibitory signal which reduces the proliferation of these CD8+ T cells at the lymph nodes and supplementary to that PD-1 is also able to control the accumulation of foreign antigen specific T cells in the lymph nodes through apoptosis which is further mediated by a lower regulation of the gene Bcl-2.

Research Category :

Autoimmune, Cancer, Cell Type Markers, Immune System Diseases, Immunology, Tumor Antigens, Tumor Biomarkers, Tumor Immunology, Tumor-Associated Antigens