Human ADA/Adenosine Deaminase ELISA Kit PicoKine® | Boster EK1446 product information

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product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human ADA/Adenosine Deaminase ELISA Kit PicoKine®

catalog :

EK1446

quantity :

96 wells/kit, with removable strips.

product information

SKU :

EK1446

Product Name :

Human ADA/Adenosine Deaminase ELISA Kit PicoKine®

Size :

96 wells/kit, with removable strips.

Description :

Human ADA/Adenosine Deaminase ELISA Kit PicoKine® (96 Tests). Quantitate Human ADA in cell culture supernatants, serum and pleural fluid. Sensitivity: 10pg/ml.

Reactivity :

Human

Application :

ELISA

Gene Name :

ADA

Uniprot ID :

P00813

Immunogen :

Expression system for standard: NS0; Immunogen sequence: A2-L363

Cross-reactivity :

There is no detectable cross-reactivity with other relevant proteins.

Assay Range :

31.2 pg/ml - 2,000 pg/ml

Sensitivity :

<10 pg/ml

Sample Type :

cell culture supernatants, serum and pleural fluid.

Capture/Detection Antibodies :

a monoclonal antibody from mouse, a biotinylated polyclonal antibody from goat

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Gene Full Name :

Adenosine deaminase

Synonyms :

Adenosine deaminase;3.5.4.4;Adenosine aminohydrolase;ADA;ADA1;

Protein Name :

Adenosine deaminase

Molecular Weight :

40764 MW

Protein Function :

Catalyzes the hydrolytic deamination of adenosine and 2- deoxyadenosine. Plays an important role in purine metabolism and in adenosine homeostasis. Modulates signaling by extracellular adenosine, and so contributes indirectly to cellular signaling events. Acts as a positive regulator of T-cell coactivation, by binding DPP4. Its interaction with DPP4 regulates lymphocyte- epithelial cell adhesion.

Subcellular Localization :

Cell membrane; Peripheral membrane protein; Extracellular side. Cell junction. Cytoplasmic vesicle lumen . Cytoplasm . Colocalized with DPP4 at the cell junction in lymphocyte-epithelial cell adhesion.

Tissue Specificity :

Found in all tissues, occurs in large amounts in T-lymphocytes and, at the time of weaning, in gastrointestinal tissues.

Background :

Adenosine Deaminase (also known as adenosine aminohydrolase, or ADA) is an enzyme involved in purine metabolism. It is needed for the breakdown of adenosine from food and for the turnover of nucleic acids in tissues. ADA in humans is involved in the development and maintenance of the immune system. However, ADA association has also been observed with epithelial cell differentiation, neurotransmission, and gestation maintenance. It has also been proposed that ADA, in addition to adenosine breakdown, stimulates release of excitatory amino acids and is necessary to the coupling of A1 adenosine receptors and heterotrimeric G proteins. Adenosine deaminase deficiency leads to pulmonary fibrosis, suggesting that chronic exposure to high levels of adenosine can exacerbate inflammation responses rather than suppressing them. It has also been recognized that adenosine deaminase protein and activity is upregulated in mouse hearts that overexpress HIF-1 alpha, which in part explains the attenuated levels of adenosine in HIF-1 alpha expressing hearts during ischemic stress.

Research Category :

Cancer, Cancer Metabolism, Chromatin Modifying Enzymes, Epigenetics and Nuclear Signaling, Metabolism, Metabolism Processes, Pathways And Processes, Response To Hypoxia