Human Caspase 3/CASP3 ELISA Kit PicoKine® | Boster EK1425 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human Caspase 3/CASP3 ELISA Kit PicoKine®

catalog :

EK1425

quantity :

96 wells/kit, with removable strips.

product information

SKU :

EK1425

Product Name :

Human Caspase 3/CASP3 ELISA Kit PicoKine®

Size :

96 wells/kit, with removable strips.

Description :

Human Caspase 3/CASP3 ELISA Kit PicoKine® (96 Tests). Quantitate Human CASP3 in cell culture supernatant sand serum. Sensitivity: 10pg/ml.

Reactivity :

Human

Application :

ELISA

Gene Name :

CASP3

Uniprot ID :

P42574

Immunogen :

Expression system for standard: E.coli; Immunogen sequence: S29-D175 & A183-H277

Cross-reactivity :

There is no detectable cross-reactivity with other relevant proteins.

Assay Range :

31.2 pg/ml - 2,000 pg/ml

Sensitivity :

<10 pg/ml

Sample Type :

cell culture supernatant sand serum

Capture/Detection Antibodies :

monoclonal antibody from mouse, polyclonal antibody from goat

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Gene Full Name :

Caspase-3

Synonyms :

Caspase-3;CASP-3;3.4.22.56;Apopain;Cysteine protease CPP32;CPP-32;Protein Yama;SREBP cleavage activity 1;SCA-1;Caspase-3 subunit p17;Caspase-3 subunit p12;CASP3;CPP32;

Protein Name :

Caspase-3

Molecular Weight :

31608 MW

Protein Function :

Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly (ADP-ribose) polymerase (PARP) at a '216-Asp- -Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop- helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.

Subcellular Localization :

Cytoplasm

Tissue Specificity :

Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.

Background :

Caspase-3 is a caspase protein that is encoded by the CASP3 gene. This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.

Research Category :

Alzheimer'S Disease, Apoptosis, Apoptotic Markers, Cancer, Caspases Etc, Cell Biology, Cell Death, Intracellular, Invasion/Microenvironment, Neurodegenerative Disease, Neurology Process, Neuroscience