Human CD73/NT5E/Ecto-5'-nucleotidase ELISA Kit PicoKine®

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human CD73/NT5E/Ecto-5'-nucleotidase ELISA Kit PicoKine®

catalog :

EK1422

quantity :

96 wells/kit, with removable strips.

product information

SKU :

EK1422

Product Name :

Human CD73/NT5E/Ecto-5'-nucleotidase ELISA Kit PicoKine®

Size :

96 wells/kit, with removable strips.

Description :

Human CD73/NT5E/Ecto-5'-nucleotidase ELISA Kit PicoKine® (96 Tests). Quantitate Human NT5E in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Reactivity :

Human

Application :

ELISA

Gene Name :

NT5E

Uniprot ID :

Q6NZX3

Immunogen :

Expression system for standard: CHO; Immunogen sequence: W27-K547

Cross-reactivity :

There is no detectable cross-reactivity with other relevant proteins.

Assay Range :

156 pg/ml - 10,000 pg/ml

Sensitivity :

<10 pg/ml

Sample Type :

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Capture/Detection Antibodies :

polyclonal antibody from sheep, polyclonal antibody from sheep

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Synonyms :

5'-nucleotidase;5'-NT;3.1.3.5;Ecto-5'-nucleotidase;CD73;NT5E;NT5, NTE;

Protein Name :

Ecto-5'-nucleotidase

Molecular Weight :

63368 MW

Protein Function :

Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities.

Subcellular Localization :

Cell membrane; Lipid-anchor, GPI-anchor.

Sequence Similarities :

Belongs to the 5'-nucleotidase family.

Background :

5'-nucleotidase (5'-NT), also known as ecto-5'-nucleotidase or CD73 (Cluster of Differentiation 73), is an enzyme that in humans is encoded by the NT5E gene. Ecto-5-prime-nucleotidase catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. And the enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5 occurs in a variety of immunodeficiency diseases. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. Rareallelic variants are associated with a syndrome of adult-onset calcification of joints and arteries (CALJA) affecting the iliac, femoral, and tibial arteries reducing circulation in the legs and the joints of the hands and feet causing pain.

Research Category :

Associated Proteins, Atherosclerosis, Cardiovascular, Hypoxia, Vascular Inflammation