Human CD44 PicoKine ELISA Kit | Boster EK1418 product information

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product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human CD44 PicoKine ELISA Kit

catalog :

EK1418

quantity :

96 wells/kit, with removable strips.

product information

sku :

EK1418

status :

Enabled

name :

Human CD44 PicoKine ELISA Kit

category name :

Picokine ELISA Kits

gene name :

CD44

assay range :

0.78ng/ml-50ng/ml

componentsneeded :

Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450ul of purified acetic acid or 700ul of concentrated hydrochloric acid to 1000ml H2 Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

conjugate :

description :

Detect Human CD44 with <20pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. CD44 tissue specificity: Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.

short description :

Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human CD44. 96wells/kit, with removable strips.

size :

96 wells/kit, with removable strips.

uniprot id :

P16070

host :

immunogen :

Expression system for standard: NSO; Immunogen sequence: Gln21 - Pro220

storage :

Store at 4 C for 6 months, at -20 C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

cross reactivity :

There is no detectable cross-reactivity with other relevant proteins.

sensitivity :

<20pg/ml

kit components :

50ng/tube

sample type :

cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

applications :

ELISA

reactivity :

Human

image labels :

Human CD44 PicoKine ELISA Kit standard curve

anticoagulant :

heparin or EDTA

assay procedure step 1 :

50ng/ml, 25ng/ml, 12.5ng/ml, 6.25ng/ml, 3.12ng/ml, 1.56ng/ml, 0.78ng/ml

background :

CD44 is an integral cell membrane glycoprotein with a postulated role in matrix adhesion lymphocyte activation and lymph node homing. It is contains 19 exons spanning 50 kb of genomic DNA. In humans, the CD44 antigen is encoded by the CD44 gene on Chromosome 11. The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.

research category :

Cancer, Cell Type Markers, Hematopoietic Progenitors, Immunology, Lymphoid, Mesenchymal Stem Cells, Myeloid, Stem Cells, Surface Molecules, T Lymphocytic Lineage, Tumor Biomarkers, Tumor Immunology

synonyms :

CD44 antigen;CDw44;Epican;Extracellular matrix receptor III;ECMR-III;GP90 lymphocyte homing/adhesion receptor;HUTCH-I;Heparan sulfate proteoglycan;Hermes antigen;Hyaluronate receptor;Phagocytic glycoprotein 1;PGP-1;Phagocytic glycoprotein I;PGP-I;CD44;CD44;LHR, MDU2, MDU3, MIC4;

gene full name :

CD44 antigen

molecular weight :

81538 MW

protein function :

Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. In cancer cells, may play an important role in invadopodia formation. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events.

subcellular localization :

Cell membrane; Single-pass type I membrane protein. Colocalizes with actin in membrane protrusions at wounding edges.

tissue specificity :

Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.

protein name :

CD44 antigen

sequence similarities :

Contains 1 Link domain.

intra inter assay cv :

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean(pg/ml) 1896 4142 25002 2011 3769 27132 Standard deviation 102.38 219.52 1526.7%2 138.75 244.98 1817.84 CV(%) 5.4% 5.3% 6.1% 6.9% 6.5% 6.7%

reproducibility :

Reproducibility . . To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots. Lots Lot1 (pg/ml) Lot2 (pg/ml) Lot3 (pg/ml) Lot4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1896 1710 1653 1784 1760 90.85 5.1% Sample 2 4142 4287 4199 4238 4216 53.12 1.2% Sample 3 25002 23581 25055 23252 24222 814.56 3.3% *number of samples for each test n=16.

sample data :

Concentration(ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.051 0.101 0.134 0.243 0.439 0.829 1.482 2.472

tmb incubation time :

15-25min

principle :

polyclonal antibody from goat polyclonal antibody from goat

last modified :

3/7/19 6:53