Human LOXL2/Lysyl Oxidase Like 2 ELISA Kit PicoKine® | Boster EK1391 product information

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product summary

company name :

Boster

product type :

ELISA/assay

product name :

Human LOXL2/Lysyl Oxidase Like 2 ELISA Kit PicoKine®

catalog :

EK1391

quantity :

96 wells/kit, with removable strips.

product information

SKU :

EK1391

Product Name :

Human LOXL2/Lysyl Oxidase Like 2 ELISA Kit PicoKine®

Size :

96 wells/kit, with removable strips.

Description :

Human LOXL2/Lysyl Oxidase Like 2 ELISA Kit PicoKine® (96 Tests). Quantitate Human LOXL2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Reactivity :

Human

Application :

ELISA

Gene Name :

LOXL2

Uniprot ID :

Q9Y4K0

Immunogen :

Expression system for standard: NS0; Immunogen sequence: Q26-Q774

Cross-reactivity :

There is no detectable cross-reactivity with other relevant proteins.

Assay Range :

156 pg/ml - 10,000 pg/ml

Sensitivity :

<10 pg/ml

Sample Type :

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Capture/Detection Antibodies :

polyclonal antibody from goat, polyclonal antibody from goat

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Gene Full Name :

Lysyl oxidase homolog 2

Synonyms :

Lysyl oxidase homolog 2;1.4.3.13;Lysyl oxidase-like protein 2;Lysyl oxidase-related protein 2;Lysyl oxidase-related protein WS9-14;LOXL2;

Protein Name :

Lysyl oxidase homolog 2

Molecular Weight :

86725 MW

Protein Function :

Mediates the post-translational oxidative deamination of lysine residues on target proteins leading to the formation of deaminated lysine (allysine). When secreted in extracellular matrix, promotes cross-linking of extracellular matrix proteins by mediating oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. Acts as a regulator of sprouting angiogenesis, probably via collagen IV scaffolding. When nuclear, acts as a transcription corepressor and specifically mediates deamination of trimethylated 'Lys-4' of histone H3 (H3K4me3), a specific tag for epigenetic transcriptional activation. Involved in epithelial to mesenchymal transition (EMT) via interaction with SNAI1 and participates in repression of E- cadherin, probably by mediating deamination of histone H3. Also involved in E-cadherin repression following hypoxia, a hallmark of epithelial to mesenchymal transition believed to amplify tumor aggressiveness, suggesting that it may play a role in tumor progression. Acts as a regulator of chondrocyte differentiation, probably by regulating expression of factors that control chondrocyte differentiation.

Subcellular Localization :

Secreted, extracellular space, extracellular matrix, basement membrane . Nucleus. Chromosome. Associated with chromatin. It is unclear how LOXL2 is nuclear: it contains a clear signal sequence and is predicted to localize in the extracellular medium. However, different reports confirmed the intracellular location and its key role in transcription regulation.

Tissue Specificity :

Expressed in many tissues. Highest expression in reproductive tissues, placenta, uterus and prostate. Up- regulated in a number of cancers cells and tissues.

Background :

Lysyl oxidase homolog 2 is an enzyme that in humans is encoded by the LOXL2 gene. This gene encodes a member of the lysyl oxidase gene family. The prototypic member of the family is essential to the biogenesis of connective tissue, encoding an extracellular copper-dependent amine oxidase that catalyses the first step in the formation of crosslinks in collagens and elastin. A highly conserved amino acid sequence at the C-terminus end appears to be sufficient for amine oxidase activity, suggesting that each family member may retain this function. The N-terminus is poorly conserved and may impart additional roles in developmental regulation, senescence, tumor suppression, cell growth control, and chemotaxis to each member of the family. LOXL2 can also crosslink collagen type IV and hence influence the sprouting of new blood vessels.

Research Category :

Cancer, Cytoskeleton / Ecm, Ecm Enzymes, Extracellular Matrix, Signal Transduction, Tumor Biomarkers