Mouse Endostatin ELISA Kit PicoKine® | Boster EK1376 product information

product summary

company name :

Boster

product type :

ELISA/assay

product name :

Mouse Endostatin ELISA Kit PicoKine®

catalog :

EK1376

quantity :

96 wells/kit, with removable strips.

price :

369 USD

more info or order :

Boster product webpage

citations: 2

Reference
Wang H, Ning T, Li M, Lu Z, Yan X, Peng Q, et al. Effect of endostatin on preventing postoperative progression of distant metastasis in a murine lung cancer model. Tumori. 2011;97:787-93 pubmed publisher
Zhou J, Chen R, Li Z, Zhou Q, Tang Q, He X, et al. [Nanoparticle-mediated endostatin gene therapy targeting hepatocellular carcinoma utilizing heat-inducible promoter]. Zhonghua Yi Xue Za Zhi. 2009;89:795-9 pubmed

image

image 1 :

Mouse Endostatin PicoKine ELISA Kit standard curve

product information

SKU :

EK1376

Product Name :

Mouse Endostatin ELISA Kit PicoKine®

Size :

96 wells/kit, with removable strips.

Price :

369 USD

Description :

Mouse Endostatin ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Col18a1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Reactivity :

Mouse

Application :

ELISA

Gene Name :

COL18A1

Uniprot ID :

P39061

Immunogen :

Expression system for standard: NS0; Immunogen sequence: H1591-K1774

Cross-reactivity :

There is no detectable cross-reactivity with other relevant proteins.

Assay Range :

156 pg/ml - 10,000 pg/ml

Sensitivity :

<10 pg/ml

Sample Type :

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Capture/Detection Antibodies :

monoclonal antibody from rat, polyclonal antibody from goat

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Gene Full Name :

Collagen alpha-1(XVIII) chain

Synonyms :

Collagen alpha-1 (XVIII) chain;Endostatin;Col18a1;

Protein Name :

Collagen alpha-1(XVIII) chain

Molecular Weight :

182172 MW

Protein Function :

Endostatin potently inhibits endothelial cell proliferation and angiogenesis. May inhibit angiogenesis by binding to the heparan sulfate proteoglycans involved in growth factor signaling.

Subcellular Localization :

Secreted, extracellular space, extracellular matrix .

Tissue Specificity :

Expressed in liver, kidney, lung, skeletal muscle and testis.

Sequence Similarities :

Belongs to the multiplexin collagen family.

Background :

Endostatin is a naturally-occurring 20-kDa C-terminal fragment derived from type XVIII collagen. It is reported to serve as an anti-angiogenic agent, similar to angiostatin and thrombospondin. And It is produced by proteolytic cleavage of collagen XVIII, a member of the multiplexin family that is characterized by interruptions in the triple helix creating multiple domains, by proteases such as cathepsins. Using a genomic clone as a probe for fluorescence in situ hybridization, Endostatin was mapped the COL18A1 gene to 21q22.3. By immunoprecipitation analysis using membrane fractions of human mammary epithelial cells, It showed that endostatin specifically bound to cell surface nucleolin with high affinity. Blockage of nucleolin with neutralizing antibody or knockdown of nucleolin by RNA interference countered the antiendothelial activity of endostatin and abrogated its antiangiogenic and antitumor activity in vivo.

Research Category :

Angiogenesis, Cancer, Cardiovascular, Collagen, Cytoskeleton / Ecm, Ecm Proteins, Extracellular Matrix, Invasion/Microenvironment, Signal Transduction

more info or order :

Boster product webpage