Mouse GPNMB/Osteoactivin ELISA Kit PicoKine® | Boster EK1331 product information

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product summary

company name :

Boster

product type :

ELISA/assay

product name :

Mouse GPNMB/Osteoactivin ELISA Kit PicoKine®

catalog :

EK1331

quantity :

96 wells/kit, with removable strips.

product information

SKU :

EK1331

Product Name :

Mouse GPNMB/Osteoactivin ELISA Kit PicoKine®

Size :

96 wells/kit, with removable strips.

Description :

Mouse GPNMB/Osteoactivin ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Gpnmb in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Reactivity :

Mouse

Application :

ELISA

Gene Name :

GPNMB

Uniprot ID :

Q99P91

Immunogen :

Expression system for standard: NS0; Immunogen sequence: K23-N502

Cross-reactivity :

There is no detectable cross-reactivity with other relevant proteins.

Assay Range :

156 pg/ml - 10,000 pg/ml

Sensitivity :

<10 pg/ml

Sample Type :

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)

Capture/Detection Antibodies :

monoclonal antibody from rat, polyclonal antibody from goat

Storage :

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Gene Full Name :

Transmembrane glycoprotein NMB

Synonyms :

Transmembrane glycoprotein NMB;DC-HIL;Dendritic cell-associated transmembrane protein;Osteoactivin;Gpnmb;Dchil, Hgfin, Nmb;

Protein Name :

Transmembrane glycoprotein NMB

Molecular Weight :

63676 MW

Protein Function :

Could be a melanogenic enzyme.

Subcellular Localization :

Cell membrane ; Single-pass type I membrane protein . Endomembrane system ; Single-pass type I membrane protein . Cytoplasmic vesicle membrane ; Single-pass type I membrane protein . Melanosome . Vesicular, endosomal like structures.

Tissue Specificity :

In early development, mRNA is detected at high levels in the outer layer of the retina. Later in development expression gets restricted to the retinal pigment epithelium and iris. Overexpressed in mammary cancer cells possessing a strong bone metastatic phenotype.

Sequence Similarities :

Belongs to the PMEL/NMB family.

Background :

Transmembrane glycoprotein NMB is a protein that is encoded by the GPNMB gene. In osteoblast progenitor cells, GPNMB works as a positive regulator of osteoblast differentiation during later stages of matrix maturation and mineralization that is mediated at least in part by BMP-2 in a SMAD1 dependent manner to promote osteoblast differentiation. GPNMB can enhance the repairing process in bone fracture, demonstrated by its high expression during chondrogenesis (soft callus) and osteogenesis (hard callus) compared to the intact femurs that is why Osteoactivin (OA) could be a novel therapeutic agent used to treat generalized osteoporosis or localized osteopenia during fracture repair by stimulating bone growth and regeneration. Similarly, GPNMB expression increases during osteoclast differentiation and it is functionally implicated in this process, possibly by promoting the fusion of osteoclast progenitor cells.

Research Category :

Cancer, Immunology, Innate Immunity, Macrophage / Inflamm., Tumor Immunology, Tumor-Associated Antigens